Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NOX82_RS05090 Genome accession   NZ_CP101752
Coordinates   1096807..1098510 (-) Length   567 a.a.
NCBI ID   WP_256514312.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain C12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1091807..1103510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOX82_RS05050 (NOX82_05050) - 1092035..1092751 (+) 717 WP_256514304.1 energy-coupling factor ABC transporter permease -
  NOX82_RS05055 (NOX82_05055) - 1092859..1093074 (+) 216 WP_074977495.1 hypothetical protein -
  NOX82_RS05060 (NOX82_05060) - 1093081..1093356 (-) 276 WP_116422667.1 type II toxin-antitoxin system RelE/ParE family toxin -
  NOX82_RS05065 (NOX82_05065) - 1093356..1093550 (-) 195 WP_116422666.1 stability determinant -
  NOX82_RS05070 (NOX82_05070) yacG 1093825..1094034 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  NOX82_RS05075 (NOX82_05075) coaE 1094031..1094639 (-) 609 WP_256514308.1 dephospho-CoA kinase -
  NOX82_RS05080 (NOX82_05080) pilD 1094713..1095585 (-) 873 WP_256514310.1 A24 family peptidase Machinery gene
  NOX82_RS05085 (NOX82_05085) pilC 1095586..1096803 (-) 1218 WP_256514311.1 type II secretion system F family protein Machinery gene
  NOX82_RS05090 (NOX82_05090) pilB 1096807..1098510 (-) 1704 WP_256514312.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NOX82_RS05100 (NOX82_05100) - 1098733..1099218 (+) 486 WP_305880434.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NOX82_RS05105 (NOX82_05105) - 1099278..1100465 (+) 1188 WP_256514313.1 hypothetical protein -
  NOX82_RS05110 (NOX82_05110) - 1100779..1101591 (+) 813 WP_256514314.1 serine hydrolase -
  NOX82_RS05120 (NOX82_05120) - 1101870..1102943 (+) 1074 WP_256514315.1 diguanylate cyclase -
  NOX82_RS05125 (NOX82_05125) - 1103105..1103332 (+) 228 WP_074977519.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62676.78 Da        Isoelectric Point: 5.5848

>NTDB_id=711969 NOX82_RS05090 WP_256514312.1 1096807..1098510(-) (pilB) [Pseudomonas citronellolis strain C12]
MNDQVVLSGLARQLVIAELLDEKAAQQAQLQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGIAFCDLGAVERESIPKE
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGEAIEKLFESVSGGFDDLGDVDLE
GLDIESGDTERKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDIPHETLLHEGFP
EDRIGSFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARREGFNDLRTSGLAKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=711969 NOX82_RS05090 WP_256514312.1 1096807..1098510(-) (pilB) [Pseudomonas citronellolis strain C12]
ATGAATGACCAAGTCGTGCTGTCCGGCCTCGCCCGCCAGCTGGTAATCGCTGAATTGTTAGATGAGAAGGCAGCCCAACA
GGCCCAGCTGCAAGCTCAACGCAACAAGCTGTCGCTAGTGACCTACCTAGTGCAGAACAAGCTCGTGAAGAGTCGTCCTC
TTACCGAGCTTGCAGCCGACCAGTTCGGTATCGCTTTTTGCGATTTGGGGGCCGTTGAAAGGGAAAGCATCCCGAAGGAG
CTGATCAGCGAAAAGCTGGTCCGCCAGCATCGGGTCATACCTCTGTGGCGCAGGGGCAATAAGCTATTCGTTGGCATCTC
AGATCCCACCAATCACCAAGCCATCACCGACGTCCAGTTCAGCACTGGTCTGACCACAGAAGCCATTCTGGTCGAAGACG
ATAAGCTTGGAGAAGCCATTGAGAAGCTCTTCGAGAGCGTCAGCGGTGGCTTTGACGATTTAGGTGATGTCGATCTAGAA
GGTCTGGACATAGAAAGCGGTGATACCGAGCGCAAGGAAGAGAGCATCGGCGGTGATGCCGATGATGCACCGGTGGTACG
CTTCGTCAACAAGATGCTGCTGGACGCAATCAAGGGTGGCTCTTCCGACTTACACTTCGAGCCCTACGAAAAAATCTACC
GAGTACGCTTCCGTACCGACGGCATGCTCCATGAGGTAGCCAAGCCCCCAATCCAATTGGCCAGTCGTATTTCCGCACGC
CTGAAGGTGATGGCTGGTCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGCCGCATCAAGATGCGTGTGTCCAAGAG
CAAGTCCATCGATTTCCGCGTCAACACCCTACCCACACTGTGGGGTGAAAAGATCGTAATGCGGATCCTCGATTCGGCAA
GCGCGCAGATGGGCATTGACGCCCTTGGCTATGAAGATGCGCAGAAGCAGCTCTATCTGGAGGCCCTGAAGCAACCGCAG
GGAATGATTCTAGTGACCGGTCCAACCGGTTCCGGCAAGACCGTATCTCTGTATACCGGTTTGAATATCCTTAATACTCC
CGACATCAATATCTCCACCGCCGAAGACCCAGTGGAGATCAACCTCGAGGGCATCAACCAGGTCAATGTCAATCCCCGCC
AAGGCATGGACTTCGCCCAGGCACTGCGCGCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTTGGTGAGATTCGCGAT
TTGGAAACCGCCGAGATCGCCATCAAGGCTGCGCAGACCGGCCACATGGTGATGTCCACGTTACACACCAACAGCGCCGC
TGAAACCCTGACTCGACTACTGAACATGGGCGTGGCCTCTTTCAACCTGGCCACTTCCACCAACCTGATCATCGCCCAAC
GCCTGGCGCGCAAGCTTTGCCCGCACTGCAAGAAGGAGCACGACATCCCTCACGAAACACTGCTCCACGAAGGTTTCCCC
GAGGACAGGATCGGCAGCTTCAAGCTCTACGCTCCCGTGGGGTGCGAAAATTGCAAGAATGGCTACAAGGGCCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCCGCACTGCAACGGATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAGAGAAGGCTTCAATGACCTGCGCACCTCAGGCCTAGCCAAGGCCATGCAAGGCATCACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.647

0.58
  pilB Acinetobacter baumannii D1279779

57.876

99.647

0.577
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.545
  pilB Vibrio cholerae strain A1552

50.266

99.295

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

52.03

95.591

0.497
  pilF Neisseria gonorrhoeae MS11

49.559

100

0.496
  pilB Vibrio campbellii strain DS40M4

51.201

95.414

0.489
  pilF Thermus thermophilus HB27

38.947

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.651

0.376