Detailed information NTG0711029

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NN913_RS12520
• Genome accession: NZ_CP101621
• Coordinates: 2560699..2561313 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 906
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2555699..2566313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NN913_RS12490 (NN913_12470)	rpsT	2555999..2556265 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NN913_RS12495 (NN913_12475)	holA	2556282..2557323 (-)	1042	Protein_2415	DNA polymerase III subunit delta	-
NN913_RS12500 (NN913_12480)	-	2557363..2557517 (-)	155	Protein_2416	hypothetical protein	-
NN913_RS12505 (NN913_12485)	-	2557558..2557692 (+)	135	WP_003152870.1	YqzM family protein	-
NN913_RS12510 (NN913_12490)	comEC	2557711..2560062 (-)	2352	WP_234949402.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NN913_RS12515 (NN913_12495)	-	2560063..2560632 (-)	570	WP_003152868.1	ComE operon protein 2	-
NN913_RS12520 (NN913_12500)	comEA	2560699..2561313 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
NN913_RS12525 (NN913_12505)	comER	2561372..2562193 (+)	822	WP_014305449.1	late competence protein ComER	-
NN913_RS12530 (NN913_12510)	-	2562262..2562999 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
NN913_RS12535 (NN913_12515)	rsfS	2562996..2563352 (-)	357	WP_003152864.1	ribosome silencing factor	-
NN913_RS12540 (NN913_12520)	yqeK	2563349..2563930 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NN913_RS12545 (NN913_12525)	-	2563920..2564489 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
NN913_RS12550 (NN913_12530)	yhbY	2564500..2564790 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NN913_RS12555 (NN913_12535)	aroE	2564784..2565626 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=711029 NN913_RS12520 WP_043867297.1 2560699..2561313(-) (comEA) [Bacillus velezensis strain 906]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=711029 NN913_RS12520 WP_043867297.1 2560699..2561313(-) (comEA) [Bacillus velezensis strain 906]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363