Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NLY38_RS02725 Genome accession   NZ_CP100553
Coordinates   569522..571639 (+) Length   705 a.a.
NCBI ID   WP_195881186.1    Uniprot ID   -
Organism   Pseudomonas hydrolytica strain KHPS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 552763..582869 569522..571639 within 0


Gene organization within MGE regions


Location: 552763..582869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLY38_RS02650 (NLY38_02650) - 552763..553134 (-) 372 WP_011920751.1 gamma-butyrobetaine hydroxylase-like domain-containing protein -
  NLY38_RS02655 (NLY38_02655) hslU 553216..554556 (-) 1341 WP_195881195.1 HslU--HslV peptidase ATPase subunit -
  NLY38_RS02660 (NLY38_02660) hslV 554596..555126 (-) 531 WP_004372920.1 ATP-dependent protease subunit HslV -
  NLY38_RS02665 (NLY38_02665) - 555223..555897 (-) 675 WP_195881194.1 SPOR domain-containing protein -
  NLY38_RS02670 (NLY38_02670) argS 555898..557637 (-) 1740 WP_195881193.1 arginine--tRNA ligase -
  NLY38_RS02675 (NLY38_02675) - 557809..560028 (-) 2220 WP_195881192.1 primosomal protein N' -
  NLY38_RS02680 (NLY38_02680) rpmE 560185..560397 (+) 213 WP_004372913.1 50S ribosomal protein L31 -
  NLY38_RS02685 (NLY38_02685) - 560424..561224 (+) 801 WP_195881191.1 thermonuclease family protein -
  NLY38_RS02690 (NLY38_02690) - 561221..562657 (+) 1437 WP_195881190.1 M48 family metalloprotease -
  NLY38_RS02695 (NLY38_02695) - 562762..564030 (+) 1269 WP_011920758.1 malic enzyme-like NAD(P)-binding protein -
  NLY38_RS02700 (NLY38_02700) - 564096..566507 (-) 2412 WP_195881189.1 penicillin-binding protein 1A -
  NLY38_RS02705 (NLY38_02705) comM 566689..567753 (+) 1065 WP_003246678.1 pilus assembly protein PilM Machinery gene
  NLY38_RS02710 (NLY38_02710) pilN 567753..568325 (+) 573 WP_195881188.1 type 4a pilus biogenesis protein PilN -
  NLY38_RS02715 (NLY38_02715) pilO 568322..568942 (+) 621 WP_195881187.1 type 4a pilus biogenesis protein PilO -
  NLY38_RS02720 (NLY38_02720) pilP 568942..569466 (+) 525 WP_080518515.1 type 4a pilus biogenesis lipoprotein PilP -
  NLY38_RS02725 (NLY38_02725) pilQ 569522..571639 (+) 2118 WP_195881186.1 type IV pilus secretin PilQ Machinery gene
  NLY38_RS02730 (NLY38_02730) aroK 571645..572163 (+) 519 WP_003246673.1 shikimate kinase AroK -
  NLY38_RS02735 (NLY38_02735) aroB 572339..573442 (+) 1104 WP_195881185.1 3-dehydroquinate synthase -
  NLY38_RS02740 (NLY38_02740) - 573453..575078 (+) 1626 WP_254348304.1 SPOR domain-containing protein -
  NLY38_RS02745 (NLY38_02745) gltB 575316..579764 (+) 4449 WP_011920767.1 glutamate synthase large subunit -
  NLY38_RS02750 (NLY38_02750) - 579799..581217 (+) 1419 WP_195881183.1 FAD-dependent oxidoreductase -
  NLY38_RS02755 (NLY38_02755) hemE 581336..582403 (+) 1068 WP_195881182.1 uroporphyrinogen decarboxylase -
  NLY38_RS02760 (NLY38_02760) - 582438..582869 (-) 432 WP_195881181.1 hypothetical protein -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 76403.69 Da        Isoelectric Point: 4.8514

>NTDB_id=705817 NLY38_RS02725 WP_195881186.1 569522..571639(+) (pilQ) [Pseudomonas hydrolytica strain KHPS2]
MNSSLSRLSVAVLASLLSPAILAANLQDLNVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKSRE
LGVGNARSVTIVEAKDRTRLIVNLTSLAPYSTRVEGNDLYVLVGDSSASATRSSASVPAAVAAVPSKKTYGPQPKAISNI
DFQRGEQGEGNIVITLSDASVSPDIQEQGGKIRLDFAKTELPESLRVRLDVKDFATPVQFVSATGSADKTSILIEPVGLY
DYLAYQTENKLTLSVKPLTQDDVERRKAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVP
WDQALDLVLKTKGLDKRQIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKATDMAKLFQSVTSADGTA
DSRGSITVDDRTNSIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSVRNGRWDVSGKDGAQ
IFDKDTGQRMFLGTDRIGNTTLDDGLASLPFVDMGVQNSTSGIAIGYLGSNIVLDLQLSAMEKTGNGEIVSQPKVVTSDK
ETAKILKGQEVPYQEASSSGATSTSFKEAALALEVTPQITPDNRIIMEVKVTKDAPDFANLVNGVPPLNKNEVNAKVLVN
DGETIVIGGVFSNTQTNAVEKVPFLGDLPFLGRVFRRDIVRDNKAELLVFITPRIMNNQAIAVNR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=705817 NLY38_RS02725 WP_195881186.1 569522..571639(+) (pilQ) [Pseudomonas hydrolytica strain KHPS2]
ATGAACAGCTCTCTTTCGCGTCTTAGCGTCGCCGTATTGGCCTCGCTGCTATCGCCCGCAATATTGGCGGCCAATCTTCA
GGATTTGAATGTCGCCAGTTTGCCTGGGGATCGGGTGGAGCTGAAGCTTTCCTTTGACGAGCCGGTTACTGCTCCTCGCG
GCTACACCATCGAGCAGCCGGCGCGTATCGCTCTGGACCTGCCGGGAGTTTCGAACAAGCTCGGCTCTAAGAGTCGAGAA
CTTGGGGTTGGCAATGCACGTAGCGTCACCATCGTCGAGGCTAAAGATCGCACCCGGCTGATCGTCAATCTCACCAGTCT
GGCACCATACAGTACCCGAGTTGAGGGTAATGACTTGTATGTCCTGGTTGGCGATTCCTCCGCTAGTGCAACTCGGTCGT
CGGCCTCGGTACCTGCTGCCGTAGCGGCAGTTCCGTCCAAGAAGACTTACGGCCCGCAGCCTAAAGCTATCAGCAATATC
GACTTTCAGCGTGGTGAGCAGGGTGAGGGGAATATCGTTATTACCCTTTCCGACGCATCCGTGAGCCCTGATATCCAGGA
GCAAGGCGGGAAGATCCGCTTGGATTTCGCCAAAACCGAGCTTCCCGAATCCTTGCGCGTGCGTCTGGATGTGAAGGATT
TTGCGACCCCGGTGCAATTTGTAAGCGCTACCGGCAGCGCCGACAAAACTAGCATTCTGATCGAGCCAGTCGGTCTTTAT
GATTATCTCGCTTACCAGACCGAGAACAAGCTGACCCTGAGCGTCAAACCGCTTACTCAGGACGATGTGGAGCGACGCAA
AGCTGAGCGTTTCGCTTATACCGGGGAGAAATTGTCGCTTAACTTCCAAGATATCGATGTGCGTTCGGTCCTGCAATTGA
TCGCTGATTTTACAGACCTGAACTTGGTAGCCAGTGACACCGTTGCCGGTAATATCACCTTGCGTCTGCAGAATGTGCCA
TGGGATCAGGCCCTGGATCTGGTGCTCAAGACCAAGGGGTTAGACAAGCGTCAGATTGGTAATGTATTGCTGGTCGCTCC
GGCGGATGAAATTGCCGCTCGTGAGCGGCAGGAACTGGAGTCACAAAAGCAGATTGCTGAGCTGGCTCCGCTGCGTCGGG
AGCTGATTCAGGTGAACTATGCCAAGGCTACCGATATGGCCAAGCTCTTTCAGTCGGTAACTAGCGCCGATGGTACAGCT
GACAGCAGGGGCTCCATTACAGTCGATGATCGTACCAACAGCATCATCGCGTATCAGACCCAAGAGCGCCTGGATGAATT
GCGTCGTATCGTGGCACAGCTCGATATTCCGGTGCGGCAGGTGATGATTGAAGCACGGATCGTTGAGGCCAACGTGGATT
ATGACAAGGCGCTTGGCGTACGCTGGGGCGGATCCGTACGAAACGGTCGTTGGGATGTTAGCGGCAAGGATGGTGCTCAG
ATTTTTGATAAAGACACCGGACAAAGAATGTTTCTGGGTACTGACCGCATTGGTAATACTACTCTAGATGATGGATTAGC
CAGTTTGCCGTTCGTGGATATGGGGGTGCAGAACAGTACTTCCGGTATTGCCATCGGCTATCTGGGCAGCAATATCGTGT
TGGATCTGCAGCTGTCTGCAATGGAGAAAACTGGTAACGGCGAAATTGTCTCTCAGCCGAAAGTGGTTACCTCTGATAAA
GAAACTGCGAAGATCTTGAAGGGGCAAGAGGTTCCTTATCAGGAGGCAAGTTCGAGCGGTGCGACTAGTACATCCTTCAA
AGAGGCTGCGTTGGCCTTGGAAGTGACTCCGCAGATCACTCCAGATAACCGCATTATCATGGAGGTTAAGGTCACGAAAG
ATGCTCCTGACTTCGCGAATCTGGTCAATGGTGTGCCTCCGCTTAATAAGAACGAGGTTAACGCCAAGGTACTGGTCAAC
GATGGTGAAACCATCGTGATCGGTGGCGTATTCTCCAATACCCAGACAAATGCGGTTGAGAAGGTGCCATTCTTGGGCGA
CCTGCCGTTCTTGGGGCGCGTTTTCCGTCGGGACATTGTCCGGGACAACAAGGCCGAGTTGCTGGTTTTCATCACTCCGC
GCATCATGAACAACCAAGCCATTGCCGTGAACCGATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(37-120)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(540-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.21

100

0.762
  pilQ Acinetobacter baumannii strain A118

40.242

100

0.424
  pilQ Acinetobacter baumannii D1279779

40.242

100

0.424
  comQ Acinetobacter baylyi ADP1

42.45

99.574

0.423
  pilQ Legionella pneumophila strain ERS1305867

37.758

96.17

0.363
  pilQ Legionella pneumophila strain Lp02

37.758

96.17

0.363