Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PF449_RS03230 Genome accession   NZ_CP115623
Coordinates   686452..687942 (+) Length   496 a.a.
NCBI ID   WP_002067248.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 2022CK-00340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 681452..692942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF449_RS03215 (PF449_03215) - 681816..684617 (+) 2802 WP_031959743.1 DUF2339 domain-containing protein -
  PF449_RS03220 (PF449_03220) - 684607..685524 (-) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  PF449_RS03225 (PF449_03225) - 685534..686355 (-) 822 WP_031959745.1 hypothetical protein -
  PF449_RS03230 (PF449_03230) pilB 686452..687942 (+) 1491 WP_002067248.1 type II secretion system ATPase GspE Machinery gene
  PF449_RS03235 (PF449_03235) - 687997..688428 (-) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  PF449_RS03240 (PF449_03240) - 688516..688953 (-) 438 WP_000371729.1 MarR family transcriptional regulator -
  PF449_RS03245 (PF449_03245) - 689114..689491 (-) 378 WP_000266123.1 DUF4951 domain-containing protein -
  PF449_RS03250 (PF449_03250) - 689506..690330 (-) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  PF449_RS03255 (PF449_03255) gigB 690357..690878 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  PF449_RS03260 (PF449_03260) gigA 690981..691949 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55345.28 Da        Isoelectric Point: 6.2032

>NTDB_id=705145 PF449_RS03230 WP_002067248.1 686452..687942(+) (pilB) [Acinetobacter baumannii strain 2022CK-00340]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHICHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=705145 PF449_RS03230 WP_002067248.1 686452..687942(+) (pilB) [Acinetobacter baumannii strain 2022CK-00340]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTCAATAGTCTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTC
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
AGATGACGCGCCTATTGTTCGTTTGATCAATGCGCTTCTCTCTGAGGCAATTCGTGTTGGTGCTTCAGATATCCATATTG
AAGCTTTTGAAAAAAAGCTATCAGTTCGTTTACGGGTAGATGGACAATTGCGTGAGATTGTACAACCGCGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGGCGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCTTCTTCTCATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATACTCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACATTTGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTAGAAACTGCTGAAATTGCGGTGCAGGCATCCTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAATACTGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCTTGCTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTGCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
ATTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

39.917

96.976

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367