Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   PDI73_RS03425 Genome accession   NZ_CP115479
Coordinates   712043..712693 (-) Length   216 a.a.
NCBI ID   WP_033899429.1    Uniprot ID   -
Organism   Lactococcus lactis strain EP2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707043..717693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDI73_RS03395 (PDI73_03395) - 707199..708731 (+) 1533 WP_270245783.1 Nramp family divalent metal transporter -
  PDI73_RS03400 (PDI73_03400) - 708833..709801 (+) 969 WP_003130649.1 PhoH family protein -
  PDI73_RS03405 (PDI73_03405) - 709859..710338 (+) 480 WP_058206727.1 NUDIX hydrolase -
  PDI73_RS03410 (PDI73_03410) ybeY 710490..710978 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  PDI73_RS03415 (PDI73_03415) - 710962..711417 (+) 456 WP_003130653.1 diacylglycerol kinase -
  PDI73_RS03420 (PDI73_03420) - 711431..712009 (+) 579 WP_021722515.1 nucleotidyltransferase family protein -
  PDI73_RS03425 (PDI73_03425) comFC/cflB 712043..712693 (-) 651 WP_033899429.1 ComF family protein Machinery gene
  PDI73_RS03430 (PDI73_03430) comFA 712690..714012 (-) 1323 WP_277730402.1 DEAD/DEAH box helicase Machinery gene
  PDI73_RS03435 (PDI73_03435) - 714069..714698 (+) 630 WP_003130660.1 YigZ family protein -
  PDI73_RS03440 (PDI73_03440) - 714832..715329 (+) 498 WP_003130662.1 VanZ family protein -
  PDI73_RS03445 (PDI73_03445) - 715377..716396 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  PDI73_RS03450 (PDI73_03450) - 716512..717258 (+) 747 WP_187398315.1 bifunctional metallophosphatase/5'-nucleotidase -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 25360.48 Da        Isoelectric Point: 9.3752

>NTDB_id=704777 PDI73_RS03425 WP_033899429.1 712043..712693(-) (comFC/cflB) [Lactococcus lactis strain EP2]
MIKCLLCSRSVAQNFLFSELFLLKNPQNLLCSKCQKNFEKIPEAHCPRCCKPNVHEICTDCQNWEKRGYKIRHQAIFHYN
QAMKDYFSQYKFVGDYRLYQIFEPYFKSISPKSPLVPIPISPKRLEERGFNQVTAFLQQDNFIELLEKENSVKQSSLNRK
ERLESPNPFRLKKGLKVPTKVILIDDIYTTGTTLYHASQILKEAGVHEIRSFSLCR

Nucleotide


Download         Length: 651 bp        

>NTDB_id=704777 PDI73_RS03425 WP_033899429.1 712043..712693(-) (comFC/cflB) [Lactococcus lactis strain EP2]
ATGATAAAGTGTTTGCTTTGTTCAAGAAGTGTTGCTCAAAACTTTCTATTTAGTGAACTTTTCCTACTCAAAAATCCTCA
AAATCTTTTATGTTCTAAATGTCAAAAAAATTTTGAAAAGATCCCTGAAGCTCACTGTCCTCGCTGCTGTAAGCCAAATG
TCCATGAGATCTGTACTGATTGTCAAAATTGGGAAAAAAGAGGATACAAAATCAGACATCAGGCAATTTTTCACTACAAT
CAAGCAATGAAAGATTATTTTAGCCAGTATAAATTTGTTGGAGATTATCGATTATACCAAATTTTTGAGCCCTATTTTAA
AAGTATTTCCCCAAAATCTCCTTTGGTTCCAATTCCTATTTCACCAAAAAGATTAGAGGAACGTGGTTTCAATCAAGTGA
CTGCTTTTCTTCAACAAGATAATTTTATTGAACTTCTTGAAAAAGAGAATTCGGTCAAACAATCTAGCCTTAATCGAAAA
GAACGATTGGAAAGTCCAAATCCTTTTCGATTAAAAAAAGGGCTAAAAGTCCCTACAAAAGTTATATTAATTGACGATAT
TTATACAACAGGTACCACCTTATATCACGCATCACAAATTCTAAAAGAAGCTGGTGTTCATGAAATAAGGAGCTTTTCTC
TTTGCCGTTAA

Domains

Predicted by InterProScan.

Phosphoribosyl transferase domain (Pribosyltran)

(176-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus mitis NCTC 12261

46.364

100

0.472
  comFC/cflB Streptococcus mitis SK321

45.909

100

0.468
  comFC/cflB Streptococcus pneumoniae Rx1

45

100

0.458
  comFC/cflB Streptococcus pneumoniae D39

45

100

0.458
  comFC/cflB Streptococcus pneumoniae R6

45

100

0.458
  comFC/cflB Streptococcus pneumoniae TIGR4

45

100

0.458
  comFC Lactococcus lactis subsp. cremoris KW2

67.164

62.037

0.417
  comFC Bacillus subtilis subsp. subtilis str. 168

37.229

100

0.398