Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VCASEI_RS11095 Genome accession   NZ_AP018680
Coordinates   2379232..2380920 (+) Length   562 a.a.
NCBI ID   WP_086961927.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2374232..2385920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS11075 pdhR 2375629..2376396 (-) 768 WP_086961920.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VCASEI_RS11080 ampD 2376770..2377336 (-) 567 WP_086961922.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VCASEI_RS11085 nadC 2377534..2378454 (+) 921 WP_086961924.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VCASEI_RS19775 - 2378789..2379223 (+) 435 WP_086961926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VCASEI_RS11095 pilB 2379232..2380920 (+) 1689 WP_086961927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VCASEI_RS11100 pilC 2380999..2382231 (+) 1233 WP_089110775.1 type II secretion system F family protein Machinery gene
  VCASEI_RS11105 pilD 2382274..2383167 (+) 894 WP_086961931.1 A24 family peptidase Machinery gene
  VCASEI_RS11110 coaE 2383179..2383796 (+) 618 WP_086961933.1 dephospho-CoA kinase -
  VCASEI_RS11115 zapD 2383837..2384574 (+) 738 WP_086961935.1 cell division protein ZapD -
  VCASEI_RS11120 yacG 2384672..2384875 (+) 204 WP_086961936.1 DNA gyrase inhibitor YacG -
  VCASEI_RS11125 mutT 2384945..2385346 (-) 402 WP_086961938.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62050.05 Da        Isoelectric Point: 6.1080

>NTDB_id=70331 VCASEI_RS11095 WP_086961927.1 2379232..2380920(+) (pilB) [Vibrio casei strain DSM 22364]
MNTNLASILRQADLLTHEQEQMVVSSVLNDNKSVLVACVDAGFLTSQDLLQHLCHLFALPQAHLEHYGYAEVCQQLGLRD
LITNHQVLPLQVDNHGVLIAVSDPTNTSVEDDFRFATGKQIELILADCKELEGAIRRLYGRSVNSESAQGKDITQDELAN
LVEVGSDEIQETEDLSQDEAPVSRFINQILLDAVRKGASDIHFEPYEDIYRVRLRCDGILIETNRPAPHLGRRLAARIKI
LSKLDIAERRLPQDGRIKIRLNPATAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYSDAQKKLYLDALQQPQGMI
LMTGPTGSGKTVSLYTGLRILNSPDKNISTAEDPVEINLTGINQVQVKPKIGFTFAAALRAFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSSSETVVRLSNMGIEAFNLASSLTLIIAQRLARRLCKHCKKTDNFPEALRSKFNIPNDV
VIYQATKQGCPECTGGYSGRVGIYEVMPFSTELANAVIKKASANDIEMIAVKQGMSTLRQSGIDNLKQGVTSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=70331 VCASEI_RS11095 WP_086961927.1 2379232..2380920(+) (pilB) [Vibrio casei strain DSM 22364]
ATGAACACTAACCTCGCCTCCATTTTACGCCAAGCAGATCTCTTGACTCACGAGCAAGAGCAAATGGTGGTGAGCAGCGT
TCTTAACGATAACAAAAGCGTCCTCGTGGCTTGCGTGGACGCTGGTTTTCTAACTAGCCAAGATTTATTACAACACCTTT
GCCACCTCTTTGCCCTGCCGCAAGCTCACCTTGAACATTACGGTTATGCCGAAGTTTGCCAACAACTGGGTTTACGAGAC
TTAATCACCAATCACCAAGTTCTGCCATTGCAAGTGGACAACCACGGTGTACTGATTGCGGTATCCGACCCCACCAACAC
CAGTGTCGAAGATGATTTCCGCTTTGCCACCGGCAAACAAATTGAGTTAATCCTTGCCGATTGCAAAGAGCTAGAAGGCG
CCATTCGCCGTTTGTACGGTCGCAGTGTGAATTCAGAGAGTGCACAAGGCAAAGACATTACCCAAGATGAGCTGGCCAAC
CTCGTTGAAGTCGGCAGTGATGAAATACAAGAAACCGAAGATTTAAGCCAAGATGAAGCGCCAGTCAGCCGATTCATTAA
CCAGATTTTACTCGATGCGGTGCGCAAAGGCGCTTCTGATATTCACTTTGAACCGTATGAAGACATTTACCGTGTTCGTC
TGCGTTGCGATGGTATCTTAATAGAAACCAACCGCCCAGCGCCACACTTAGGCCGACGTTTAGCCGCAAGGATTAAAATA
TTATCCAAGCTGGATATTGCCGAGCGCCGCCTCCCCCAAGATGGCCGAATAAAAATTCGCTTGAACCCCGCAACGGCCAT
CGACATGCGAGTATCAACGCTCCCAACATTGTGGGGGGAAAAAATTGTATTGCGCTTACTCGACAGCAGCGCCGCCAATC
TCAATATTGATAAACTCGGCTACAGTGACGCGCAAAAGAAATTGTACCTCGACGCCCTGCAACAACCGCAAGGCATGATT
TTAATGACCGGTCCAACCGGAAGCGGCAAAACGGTATCACTTTATACTGGGCTACGCATCTTAAACTCACCGGATAAAAA
TATTTCCACCGCAGAAGATCCCGTTGAAATCAACTTAACCGGCATCAATCAGGTACAAGTAAAACCAAAAATAGGTTTTA
CCTTTGCGGCTGCTTTACGGGCATTTTTACGCCAAGATCCCGATGTGGTAATGGTGGGTGAAATTCGTGATTTAGAAACC
GCCGAAATAGCCATAAAAGCAGCGCAAACTGGGCACTTAGTACTATCAACCTTGCACACTAATTCGTCATCAGAAACCGT
GGTGCGCTTATCTAACATGGGGATTGAAGCCTTTAATTTAGCTTCTTCATTAACCTTAATCATTGCCCAACGCCTCGCCC
GTCGTTTATGCAAACATTGTAAAAAAACCGATAACTTCCCAGAAGCATTGCGCAGCAAATTCAATATTCCTAATGATGTG
GTGATTTACCAAGCCACTAAACAAGGCTGCCCAGAATGTACCGGGGGTTACTCAGGTCGGGTCGGTATTTATGAAGTCAT
GCCTTTTAGCACCGAGCTGGCTAATGCCGTTATTAAAAAAGCATCGGCAAACGACATTGAAATGATTGCGGTAAAGCAAG
GCATGTCGACTCTGCGCCAATCAGGAATCGACAACTTGAAGCAAGGAGTCACCAGTTTTGCGGAATTACAACGCGTACTG
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

69.573

100

0.696
  pilB Vibrio parahaemolyticus RIMD 2210633

68.683

100

0.687
  pilB Vibrio campbellii strain DS40M4

68.149

100

0.681
  pilB Acinetobacter baylyi ADP1

48.502

95.018

0.461
  pilB Acinetobacter baumannii D1279779

50.685

90.925

0.461
  pilB Legionella pneumophila strain ERS1305867

47.858

95.552

0.457
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.445
  pilF Thermus thermophilus HB27

38.547

95.552

0.368

Multiple sequence alignment