Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NHL60_RS01100 Genome accession   NZ_CP099975
Coordinates   221994..223136 (+) Length   380 a.a.
NCBI ID   WP_253447873.1    Uniprot ID   -
Organism   Halomonas sp. Y3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 216994..228136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHL60_RS01075 - 217399..218556 (-) 1158 WP_253447856.1 homoserine O-acetyltransferase -
  NHL60_RS01080 - 218609..219190 (-) 582 WP_253447859.1 YggT family protein -
  NHL60_RS01085 proC 219249..220073 (-) 825 WP_253447862.1 pyrroline-5-carboxylate reductase -
  NHL60_RS01090 - 220144..220863 (-) 720 WP_253447865.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHL60_RS01095 pilT 220930..221967 (+) 1038 WP_253447869.1 type IV pilus twitching motility protein PilT Machinery gene
  NHL60_RS01100 pilU 221994..223136 (+) 1143 WP_253447873.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHL60_RS01105 - 223156..224295 (-) 1140 WP_253447875.1 anhydro-N-acetylmuramic acid kinase -
  NHL60_RS01110 tyrS 224411..225610 (+) 1200 WP_253447878.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42378.74 Da        Isoelectric Point: 6.4356

>NTDB_id=703080 NHL60_RS01100 WP_253447873.1 221994..223136(+) (pilU) [Halomonas sp. Y3]
MMAKEWLYQLLDIMVQQEASDLLISVNAPPTLKRAGQLIPMGDQRLSVEQVNELVNIAIPVGMAERFQLEREANFALSLK
DRGRFRVSAFQQRNQKAMVIRRIAFEIPHLETLGLPGVLGELANLKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTDSFEVALKNTLRQAPDVVLIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLRAIVAQQLLPTVDGGRCAAIEILLRSPLISDLIGKGDVSEIKRVMGRSRDQGMQTF
DQALYDLFRAGRITEEVALVHADSANDLRMMIKYGDEGSEGLAQARKAASQLSLKRDEDF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=703080 NHL60_RS01100 WP_253447873.1 221994..223136(+) (pilU) [Halomonas sp. Y3]
ATGATGGCGAAGGAGTGGCTCTATCAGCTGCTGGACATCATGGTGCAGCAGGAGGCGTCGGATCTGCTGATCTCGGTCAA
TGCACCGCCGACGCTCAAGCGGGCCGGTCAGCTGATTCCCATGGGAGATCAGCGGCTTTCCGTGGAGCAGGTCAACGAGC
TGGTCAATATCGCCATTCCCGTGGGCATGGCCGAGCGCTTTCAGCTGGAGCGCGAGGCCAACTTCGCGCTTAGCCTCAAG
GACAGGGGGCGCTTTCGTGTCAGCGCCTTTCAGCAGCGCAACCAGAAGGCCATGGTGATTCGCCGAATCGCCTTCGAGAT
TCCTCACCTGGAGACGCTCGGGCTGCCGGGCGTGCTGGGCGAGCTGGCCAACCTCAAGCGCGGTCTGGTGTTCGTGGTGG
GGGGGACCGGTACCGGCAAGTCGACCACCCTGGCCTCGATGATCCAGCAGCGTAACGAGACCCTGGGGGGCCATATCATC
AGCGTCGAGGACCCCATCGAGTATGTGCATCCGCACAAGAAGGCGATCATCAACCAGCGTGAGGTAGGCATCGATACCGA
CTCCTTCGAGGTGGCGCTGAAGAACACCCTACGCCAGGCTCCTGACGTCGTGCTGATCGGCGAGATCCGGACCCGGGAAA
CCATGGAGCATGCCCTGACCTTTGCCGAGACCGGCCACCTCTGCCTGGCGACCCTGCATGCCAACAACGCCAACCAGGCA
CTGGACCGCATCATTCACTTCTTCCCCCACGAGCGCCACGAGCAGATCTGGCTGGACCTTTCCCTCAACCTGCGTGCCAT
CGTGGCCCAGCAGCTGCTGCCCACGGTGGACGGTGGCCGCTGTGCGGCCATCGAGATCCTGCTCAGGTCGCCACTGATCA
GCGACCTGATCGGCAAGGGAGATGTCAGCGAGATCAAGCGGGTGATGGGGCGCTCCCGGGATCAGGGCATGCAGACCTTT
GATCAGGCGCTCTACGACCTCTTTCGGGCGGGGAGGATCACCGAGGAGGTGGCGCTGGTGCACGCCGATTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGCGATGAGGGTAGCGAGGGGCTGGCCCAGGCGCGCAAGGCGGCGAGCCAGCTCTCGC
TCAAGCGCGACGAGGACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62

92.105

0.571
  pilU Vibrio cholerae strain A1552

56.868

95.789

0.545
  pilU Acinetobacter baylyi ADP1

54.93

93.421

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.71

100

0.424
  pilT Neisseria meningitidis 8013

41.141

87.632

0.361