Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NI376_RS02595 Genome accession   NZ_CP099943
Coordinates   566156..567832 (+) Length   558 a.a.
NCBI ID   WP_222242591.1    Uniprot ID   -
Organism   Pseudoalteromonas piscicida strain BM7A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561156..572832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI376_RS02575 - 562658..563119 (+) 462 WP_308374103.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NI376_RS02580 (NI376_02575) pilC 563186..564409 (+) 1224 WP_308374734.1 type II secretion system F family protein Machinery gene
  NI376_RS02585 (NI376_02580) pilD 564543..565421 (+) 879 WP_308374104.1 A24 family peptidase Machinery gene
  NI376_RS02590 (NI376_02585) coaE 565431..566060 (+) 630 WP_308374105.1 dephospho-CoA kinase -
  NI376_RS02595 (NI376_02590) pilB 566156..567832 (+) 1677 WP_222242591.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI376_RS02600 (NI376_02595) rlmM 568119..569204 (-) 1086 WP_010607344.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  NI376_RS02605 (NI376_02600) - 569218..569586 (-) 369 WP_017217933.1 DUF423 domain-containing protein -
  NI376_RS02610 (NI376_02605) - 569579..570217 (-) 639 WP_045989837.1 alpha/beta family hydrolase -
  NI376_RS02615 (NI376_02610) - 570214..571119 (-) 906 WP_010370478.1 transcriptional regulator GcvA -
  NI376_RS02620 (NI376_02615) kdsA 571205..572059 (-) 855 WP_010370475.1 3-deoxy-8-phosphooctulonate synthase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62047.34 Da        Isoelectric Point: 5.7022

>NTDB_id=702755 NI376_RS02595 WP_222242591.1 566156..567832(+) (pilB) [Pseudoalteromonas piscicida strain BM7A]
MEHHSPLLRKFITLGRVTAEQIKSRQPEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCEHCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=702755 NI376_RS02595 WP_222242591.1 566156..567832(+) (pilB) [Pseudoalteromonas piscicida strain BM7A]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTCACTGCTGAGCAAATAAAGTCTCGCCAGCC
AGAAGCAAACACCACCGCCGAGTTAATTTGCCTAAGTTCAGGAATGAGTTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTCAAGGTGCCCTTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCGGCGCTAGTCAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTACGTTGCGGCTTCTGATCCTACTGACTTTGGCGC
ATTTGAAAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTACTGGTAGCCGACTACAAAAAACTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGCCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGATAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTACACTTTGAGCCCTACGAGAAGAAATATCGCGTGCGCTTTCGTATAGATGGCCTGC
TGCATGAGATGGCAAGTCCACCAAACACACTAGCAACTCGCCTCGCCGCCCGTATCAAAGTGATGGCGCACTTAGACATA
GCGGAAAAACGCAAACCGCAAGATGGCCGCATTAAATTAAAGATCTCCGAGCGCAAAAGTATTGATTTTCGTGTGAGCAC
GCTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATTGACGTGCTAG
GTTATGAGCCTGAGCAAAAACAACTTTACCTCGACGCACTAGAACAGCCTCAGGGCATGATTTTGGTAACCGGTCCAACG
GGTTCCGGTAAAACCGTCTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACCGCGGAAGA
CCCTGTCGAAATAAACCTTGAGGGTATTAACCAAGTACAAATCAATACCAAAGCGGATATGACTTTCGCCAATGCCTTGA
GAGCATTCTTACGTCAGGATCCGGATGTAGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAAATATCAATAAAA
GCCGCCCAAACCGGTCACTTGGTATTGAGTACACTGCACACCAACTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCGTACAACGTAGCAAGCTCGGTGAGTTTGATTATTGCCCAGCGTCTCGCAAGACGCCTTTGCCCTAAGT
GTAAAACACCTGAAACGCTGCCAGAAGAAGCCTTGTTAGGGCAAGGCTTTACTGCCGAGCAAATTAAAGAGATTACCCTT
TTTGCGCCTAAAGGCTGTGAACACTGCACCGATGGTTACAAAGGCCGTGTGGGCATTTATGAAGTGGTAAAAATCACGCC
AGAGCTTTCGCACCTGATCATGGAAGGGGGAAACTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAATCTGGCCTGAAAAAAGCCGCCGCTGGAGTGACATCGCTTACAGAAATCAACCGCGTAACGAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

53.005

98.387

0.522
  pilB Vibrio campbellii strain DS40M4

52.545

98.566

0.518
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362