Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NI389_RS06815 Genome accession   NZ_CP099917
Coordinates   1478947..1480626 (+) Length   559 a.a.
NCBI ID   WP_308362140.1    Uniprot ID   -
Organism   Pseudoalteromonas xiamenensis strain PSDB     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1473947..1485626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI389_RS06790 (NI389_06775) nadC 1474109..1474966 (+) 858 WP_308362135.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI389_RS06795 (NI389_06780) - 1475592..1476029 (+) 438 WP_308362136.1 pilin -
  NI389_RS06800 (NI389_06785) pilC 1476080..1477309 (+) 1230 WP_308362137.1 type II secretion system F family protein Machinery gene
  NI389_RS06805 (NI389_06790) pilD 1477331..1478230 (+) 900 WP_308362138.1 A24 family peptidase Machinery gene
  NI389_RS06810 (NI389_06795) coaE 1478227..1478841 (+) 615 WP_308362139.1 dephospho-CoA kinase -
  NI389_RS06815 (NI389_06800) pilB 1478947..1480626 (+) 1680 WP_308362140.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI389_RS06820 (NI389_06805) yacG 1480719..1480961 (+) 243 WP_308362141.1 DNA gyrase inhibitor YacG -
  NI389_RS06830 (NI389_06815) - 1481563..1482059 (+) 497 Protein_1330 RDD family protein -
  NI389_RS06835 (NI389_06820) lptG 1482138..1483205 (-) 1068 WP_308362142.1 LPS export ABC transporter permease LptG -
  NI389_RS06840 (NI389_06825) lptF 1483202..1484314 (-) 1113 WP_308362143.1 LPS export ABC transporter permease LptF -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62128.51 Da        Isoelectric Point: 5.9689

>NTDB_id=702524 NI389_RS06815 WP_308362140.1 1478947..1480626(+) (pilB) [Pseudoalteromonas xiamenensis strain PSDB]
MEQNSPLLRKFLTLGRVTAEQVKAKQKDASSIAELIAMCSGMDGKELAEQCLDLFRVPLFDLNEFDIENIPKELVKEKLI
RQHHILPLNRKGRKLYVAASDPTDYGALENFEFSTGLQCEVVVVDYRQLEKKIDLLFDSSSGLGMSEAEFDEFSSLETDD
SSTVQAQQEEEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEMASPPVTLANRLSARIKVMSRLD
IAEKRKPQDGRIKLKISDRKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALNQPQGMILVTGP
TGSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISI
KAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPKEELLRQGFKEADIPNVT
LFSPKGCDHCTDGYKGRVGIYEVVKITPEIAQVIMRGGNSLEIAEMANKAGFANLRISGLKKAGAGVSSLTEINRVTSF

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=702524 NI389_RS06815 WP_308362140.1 1478947..1480626(+) (pilB) [Pseudoalteromonas xiamenensis strain PSDB]
ATGGAGCAAAACTCCCCATTATTACGTAAGTTCTTAACATTAGGACGCGTGACCGCTGAGCAGGTTAAAGCAAAGCAAAA
AGATGCAAGTTCAATTGCAGAGTTAATTGCGATGTGCAGCGGCATGGATGGCAAAGAGTTAGCCGAACAATGCCTAGATC
TATTTCGAGTTCCCTTATTCGATTTGAATGAGTTTGATATCGAAAACATTCCAAAAGAACTTGTTAAAGAAAAGCTCATT
CGACAACATCATATTCTTCCACTTAACCGTAAAGGACGGAAATTGTACGTCGCCGCGTCTGATCCAACTGATTATGGCGC
CCTCGAAAACTTTGAGTTCAGTACAGGCCTTCAATGTGAAGTTGTCGTTGTCGATTATCGTCAACTTGAAAAGAAAATTG
ACTTATTGTTCGACTCATCAAGTGGACTTGGCATGTCGGAAGCTGAGTTTGACGAATTTAGTTCACTTGAAACAGATGAC
TCTTCAACCGTTCAAGCACAACAGGAAGAAGAAAAAGACGATGCACCTATCATCGTCTACATCAATAAGATACTGATGGA
TGCCATCAAAAAAGGTGCATCGGATCTTCACTTCGAACCGTACGAGCACAAGTACCGAATTCGATTTCGTATCGATGGTA
TTTTACATGAAATGGCAAGTCCACCGGTTACGCTCGCAAACCGACTTTCTGCCCGTATCAAGGTTATGTCTCGTCTAGAT
ATTGCAGAAAAGCGTAAACCTCAAGACGGTCGCATTAAACTCAAAATATCGGATAGGAAAAGCATCGATTTCCGTGTGAG
CACTTTGCCAACCATGTGGGGCGAAAAAATAGTAATGCGTATTCTGGATTCTTCCAGCGCAATGCTCGGCATCGATGTTT
TAGGATATGAACCTGAACAAAAGCAGCTTTACCTTGACGCACTCAATCAACCTCAAGGGATGATTTTGGTCACTGGGCCG
ACCGGCTCCGGTAAAACTGTTTCACTTTACACGGGTTTAAACATTCTCAACAAACCAGAGCGTAACATCAGTACCGCTGA
AGACCCTGTTGAAATAAACCTTGAAGGTATTAACCAAGTTCAAATCAATACCAAGGCTGATATGACATTCGCTAATGCGC
TGCGTGCATTCCTACGTCAAGATCCTGATGTGGTGATGGTGGGTGAGATCCGAGATTTGGAAACGGCTGAAATTTCCATT
AAAGCGGCACAAACAGGTCACTTGGTGCTAAGTACACTTCACACCAACTCTGCCCCCGAAACACTCACTCGTTTACTCAA
TATGGGCGTGCCTGCCTACAACGTTGCCAGCTCTGTCAGTTTGATTATCGCACAACGCCTTGCTCGTCGTTTATGCCCTA
AATGTAAAACACCAGAAACATTGCCGAAAGAAGAGCTCCTAAGACAAGGCTTCAAAGAAGCGGATATCCCAAATGTAACG
CTGTTTTCGCCAAAAGGCTGCGATCATTGTACCGATGGCTACAAAGGCCGAGTCGGTATATACGAAGTAGTAAAAATCAC
TCCTGAAATAGCACAGGTGATTATGCGAGGTGGTAATAGTCTTGAAATTGCCGAGATGGCAAACAAAGCTGGATTTGCGA
ATCTGCGTATTTCTGGCCTCAAAAAAGCAGGAGCTGGCGTCAGTTCTCTTACCGAAATTAACCGCGTCACCAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.63

96.601

0.576
  pilB Acinetobacter baylyi ADP1

56.406

100

0.567
  pilB Vibrio cholerae strain A1552

54.098

98.211

0.531
  pilB Vibrio campbellii strain DS40M4

53.223

97.138

0.517
  pilB Vibrio parahaemolyticus RIMD 2210633

53.223

97.138

0.517
  pilB Legionella pneumophila strain ERS1305867

56.496

90.877

0.513
  pilF Neisseria gonorrhoeae MS11

47.679

100

0.478
  pilB/pilB1 Synechocystis sp. PCC 6803

37.825

100

0.417