Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCP57_RS10705 Genome accession   NZ_CP115287
Coordinates   2314410..2315819 (+) Length   469 a.a.
NCBI ID   WP_003120811.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309410..2320819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP57_RS10690 (PCP57_10695) gspL 2310214..2311425 (+) 1212 WP_328995065.1 type II secretion system protein GspL -
  PCP57_RS10695 (PCP57_10700) - 2311412..2312005 (+) 594 WP_033973122.1 type II secretion system protein M -
  PCP57_RS10700 (PCP57_10705) gspD 2312002..2314413 (+) 2412 WP_328995068.1 type II secretion system secretin GspD -
  PCP57_RS10705 (PCP57_10710) pilB 2314410..2315819 (+) 1410 WP_003120811.1 type II secretion system ATPase GspE Machinery gene
  PCP57_RS10710 (PCP57_10715) pilC 2315819..2317033 (+) 1215 WP_022579875.1 type II secretion system inner membrane protein GspF Machinery gene
  PCP57_RS10715 (PCP57_10720) - 2317546..2318652 (+) 1107 WP_033973119.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50994.41 Da        Isoelectric Point: 5.7943

>NTDB_id=702001 PCP57_RS10705 WP_003120811.1 2314410..2315819(+) (pilB) [Pseudomonas aeruginosa strain F006]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=702001 PCP57_RS10705 WP_003120811.1 2314410..2315819(+) (pilB) [Pseudomonas aeruginosa strain F006]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGTACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAA
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACCGACCTGCTCGACACCCAGGATGGCGCGCCGGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCGGCGCGCGACGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGATATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCCCCGGGCGTGCTGGCGCCGCTGGATAACCTGATCCGCCAGCCGCACGGT
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCGCTGGCACGGCTCGACGCCAGCAC
CAGCAATATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGACACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCACTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGTCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAGCTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373