Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NHF44_RS03695 Genome accession   NZ_CP099743
Coordinates   749855..750835 (+) Length   326 a.a.
NCBI ID   WP_004149811.1    Uniprot ID   A6TDW0
Organism   Klebsiella pneumoniae strain 503666-2-2019     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744855..755835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHF44_RS03665 (NHF44_03665) - 745186..746232 (-) 1047 WP_004185615.1 sugar ABC transporter substrate-binding protein -
  NHF44_RS03670 (NHF44_03670) hemW 746525..747664 (-) 1140 WP_016529066.1 radical SAM family heme chaperone HemW -
  NHF44_RS03675 (NHF44_03675) - 747657..748250 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  NHF44_RS03680 (NHF44_03680) yggU 748263..748553 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  NHF44_RS03685 (NHF44_03685) - 748550..749116 (-) 567 WP_002916613.1 YggT family protein -
  NHF44_RS03690 (NHF44_03690) - 749137..749838 (-) 702 WP_004144780.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHF44_RS03695 (NHF44_03695) pilT 749855..750835 (+) 981 WP_004149811.1 type IV pilus twitching motility protein PilT Machinery gene
  NHF44_RS03700 (NHF44_03700) - 751184..751837 (+) 654 WP_002916607.1 LuxR family transcriptional regulator -
  NHF44_RS03705 (NHF44_03705) ruvX 751890..752306 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  NHF44_RS03710 (NHF44_03710) - 752306..752869 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  NHF44_RS03715 (NHF44_03715) gshB 753044..753994 (-) 951 WP_023286872.1 glutathione synthase -
  NHF44_RS03720 (NHF44_03720) rsmE 754015..754746 (-) 732 WP_002916597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NHF44_RS03725 (NHF44_03725) endA 754838..755545 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35327.83 Da        Isoelectric Point: 6.8575

>NTDB_id=701464 NHF44_RS03695 WP_004149811.1 749855..750835(+) (pilT) [Klebsiella pneumoniae strain 503666-2-2019]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALNLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDMLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=701464 NHF44_RS03695 WP_004149811.1 749855..750835(+) (pilT) [Klebsiella pneumoniae strain 503666-2-2019]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGAACCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGTCTCCTTCCTGAACAGTGCCCGCGTCTGGATATGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6TDW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

40.187

98.466

0.396