Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NHU86_RS02755 Genome accession   NZ_CP099742
Coordinates   570745..571854 (-) Length   369 a.a.
NCBI ID   WP_113069594.1    Uniprot ID   -
Organism   Aeromonas caviae strain OT55     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 565745..576854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHU86_RS02730 (NHU86_02725) - 565878..566105 (+) 228 WP_039039956.1 hypothetical protein -
  NHU86_RS02735 (NHU86_02730) brnQ 566195..567457 (-) 1263 WP_235671999.1 branched-chain amino acid transport system II carrier protein -
  NHU86_RS02740 (NHU86_02735) - 567693..568403 (-) 711 WP_113069592.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NHU86_RS02745 (NHU86_02740) srmB 568531..569751 (+) 1221 WP_113069593.1 ATP-dependent RNA helicase SrmB -
  NHU86_RS02750 (NHU86_02745) yaaA 569939..570712 (-) 774 WP_039039959.1 peroxide stress protein YaaA -
  NHU86_RS02755 (NHU86_02750) pilU 570745..571854 (-) 1110 WP_113069594.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHU86_RS02760 (NHU86_02755) pilT 571878..572912 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  NHU86_RS02765 (NHU86_02760) - 572952..573653 (+) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHU86_RS02770 (NHU86_02765) proC 573783..574607 (+) 825 WP_069784313.1 pyrroline-5-carboxylate reductase -
  NHU86_RS02775 (NHU86_02770) - 574631..575182 (+) 552 WP_113069595.1 YggT family protein -
  NHU86_RS02780 (NHU86_02775) yggU 575182..575481 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  NHU86_RS02785 (NHU86_02780) - 575500..575919 (+) 420 WP_029313762.1 DUF4426 domain-containing protein -
  NHU86_RS02790 (NHU86_02785) - 575995..576825 (-) 831 WP_113069596.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40867.97 Da        Isoelectric Point: 6.2677

>NTDB_id=701441 NHU86_RS02755 WP_113069594.1 570745..571854(-) (pilU) [Aeromonas caviae strain OT55]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGAGTLDNVTLDD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=701441 NHU86_RS02755 WP_113069594.1 570745..571854(-) (pilU) [Aeromonas caviae strain OT55]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGCTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCACGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379