Detailed information NTG0701441

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCP35_RS00580
• Genome accession: NZ_CP115278
• Coordinates: 130755..131819 (+)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain F011
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 125755..136819

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP35_RS00570 (PCP35_00575)	-	126726..127994 (+)	1269	WP_004347579.1	malic enzyme-like NAD(P)-binding protein	-
PCP35_RS00575 (PCP35_00580)	-	128099..130570 (-)	2472	WP_003095841.1	penicillin-binding protein 1A	-
PCP35_RS00580 (PCP35_00585)	pilM	130755..131819 (+)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PCP35_RS00585 (PCP35_00590)	pilN	131819..132415 (+)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
PCP35_RS00590 (PCP35_00595)	pilO	132412..133035 (+)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
PCP35_RS00595 (PCP35_00600)	comL/pilP	133032..133556 (+)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PCP35_RS00600 (PCP35_00605)	pilQ	133610..135754 (+)	2145	WP_023114914.1	type 4a pilus secretin PilQ	Machinery gene
PCP35_RS00605 (PCP35_00610)	aroK	135766..136284 (+)	519	WP_003095833.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=701441 PCP35_RS00580 WP_003110888.1 130755..131819(+) (pilM) [Pseudomonas aeruginosa strain F011]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=701441 PCP35_RS00580 WP_003110888.1 130755..131819(+) (pilM) [Pseudomonas aeruginosa strain F011]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466