Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PCP64_RS03385 Genome accession   NZ_CP115276
Coordinates   709237..709701 (-) Length   154 a.a.
NCBI ID   WP_021205141.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704237..714701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP64_RS03370 (PCP64_03370) nadC 706747..707595 (+) 849 WP_047688821.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP64_RS03380 (PCP64_03380) - 707776..709155 (-) 1380 WP_236080397.1 O-antigen ligase family protein -
  PCP64_RS03385 (PCP64_03385) pilA/pilAI 709237..709701 (-) 465 WP_021205141.1 pilin Machinery gene
  PCP64_RS03390 (PCP64_03390) pilB 709932..711632 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP64_RS03395 (PCP64_03395) pilC 711636..712853 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PCP64_RS03400 (PCP64_03400) pilD 712854..713726 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP64_RS03405 (PCP64_03405) coaE 713723..714334 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP64_RS03410 (PCP64_03410) yacG 714331..714531 (+) 201 WP_060962072.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16076.44 Da        Isoelectric Point: 9.0145

>NTDB_id=701243 PCP64_RS03385 WP_021205141.1 709237..709701(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F013]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=701243 PCP64_RS03385 WP_021205141.1 709237..709701(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F013]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCTAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.945

94.805

0.455
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37