Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NF675_RS01930 Genome accession   NZ_CP099596
Coordinates   448144..450231 (+) Length   695 a.a.
NCBI ID   WP_056787342.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6BAGNBB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443144..455231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF675_RS01910 (NF675_01910) pilM 445359..446423 (+) 1065 WP_056787331.1 pilus assembly protein PilM Machinery gene
  NF675_RS01915 (NF675_01915) - 446423..446989 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF675_RS01920 (NF675_01920) pilO 446986..447609 (+) 624 WP_252879288.1 type 4a pilus biogenesis protein PilO -
  NF675_RS01925 (NF675_01925) - 447606..448130 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  NF675_RS01930 (NF675_01930) pilQ 448144..450231 (+) 2088 WP_056787342.1 type IV pilus secretin PilQ Machinery gene
  NF675_RS01935 (NF675_01935) aroK 450236..450754 (+) 519 WP_252879289.1 shikimate kinase AroK -
  NF675_RS01940 (NF675_01940) aroB 450907..452007 (+) 1101 WP_041477573.1 3-dehydroquinate synthase -
  NF675_RS01945 (NF675_01945) - 452018..453604 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75138.44 Da        Isoelectric Point: 5.4106

>NTDB_id=700862 NF675_RS01930 WP_056787342.1 448144..450231(+) (pilQ) [Pseudomonas siliginis strain OTU6BAGNBB1]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQMANKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATAATAAAAPARPAAQRSR
AIRGVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVE
PSGTFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNAS
GVNGSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=700862 NF675_RS01930 WP_056787342.1 448144..450231(+) (pilQ) [Pseudomonas siliginis strain OTU6BAGNBB1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGATGGCCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGCAGCAGG
GCGATTCGCGGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAATGTGGTCATCGATCTCTCTGATCCGACCATCGC
CCCGGATATCCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCC
TCGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAG
CCCAGCGGCACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGGCCGATGAGCATCGACGA
CCTGCAAAAGCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGTT
CGGTACTGCAACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACTTTGCGC
CTGCAGAACGTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGT
GCTGCTGGTCGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGTTGG
CGCCCCTGCGTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGC
GCCGAGGCCAAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGA
TCGCCTCGACGAACTCCGGCGGATCGTCGCCCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTTG
AGGCCAACGTCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCC
GGGGTCAACGGTTCGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGT
CAATAACACTTCGGGGATCGGCATTGCTTTCATCACCGACAACGTACTGCTGGATCTCGAACTGACGGCGATGGAAAAAA
CCGGCAATGGCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAG
ATTCCCTATCAGGAGGCCAGCTCCAGCGGCGCTACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCC
GCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTT
TTTTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.688

100

0.74
  comQ Acinetobacter baylyi ADP1

41.32

100

0.414
  pilQ Acinetobacter baumannii strain A118

37.366

100

0.4
  pilQ Acinetobacter baumannii D1279779

37.366

100

0.4
  pilQ Legionella pneumophila strain Lp02

37.83

98.129

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.794

97.842

0.37