Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NGM67_RS18045 Genome accession   NZ_CP099545
Coordinates   2823752..2825443 (+) Length   563 a.a.
NCBI ID   WP_252689979.1    Uniprot ID   -
Organism   Photobacterium damselae strain XP-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2818752..2830443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM67_RS18030 (NGM67_18030) pdhR 2821060..2821833 (-) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NGM67_RS18035 (NGM67_18035) ampD 2822193..2822771 (-) 579 WP_068945541.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NGM67_RS18040 (NGM67_18040) - 2823255..2823752 (+) 498 WP_252689978.1 pilin -
  NGM67_RS18045 (NGM67_18045) pilB 2823752..2825443 (+) 1692 WP_252689979.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGM67_RS18050 (NGM67_18050) pilC 2825509..2826735 (+) 1227 WP_252689980.1 type II secretion system F family protein Machinery gene
  NGM67_RS18055 (NGM67_18055) pilD 2826918..2827796 (+) 879 WP_106142459.1 prepilin peptidase Machinery gene
  NGM67_RS18060 (NGM67_18060) coaE 2827798..2828400 (+) 603 WP_109374725.1 dephospho-CoA kinase -
  NGM67_RS18065 (NGM67_18065) zapD 2828468..2829205 (+) 738 WP_005297594.1 cell division protein ZapD -
  NGM67_RS18070 (NGM67_18070) yacG 2829214..2829423 (+) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  NGM67_RS18075 (NGM67_18075) mutT 2829621..2830061 (+) 441 WP_158135331.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62524.11 Da        Isoelectric Point: 5.2594

>NTDB_id=700576 NGM67_RS18045 WP_252689979.1 2823752..2825443(+) (pilB) [Photobacterium damselae strain XP-11]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPTG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=700576 NGM67_RS18045 WP_252689979.1 2823752..2825443(+) (pilB) [Photobacterium damselae strain XP-11]
ATGCACACCACTCTCGCCTCGATTCTGCACCAAGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGTATCTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATATTTGGTCTCACCCTCGTTTCGGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGTGCACTGCCCCTCGCTCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAACAT
CGACGGGCAAGATGAGTTCCGTTTTGCCACTGGCCGTAATATTGAACCGCTACTGCTGGAAAATAAGCAGCTCGAATCGG
CTATCCGTCGTATTTATGGTCGCGAGTTGGGCGAAACCACCAATATGGCCAGTCGTGATGTCAGCGATGATGATCTGAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCAAACCACAGATCTAGGCCAAGAAAACGAACCTGTTACTCGCTATAT
CAACCAAGTACTGCTCGATGCAGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCATATGAGACCAGCTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTTAAGCTCACAGCCAACTTAGC
CGTAGATCTGCGAGTGTCTACCCTGCCAACTTTATGGGGGGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAGCCGCAAGGGATG
ATCTTAATGACAGGGCCAACGGGCAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATCTCTACCGCTGAAGATCCAGTGGAGATCAACTTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCTGGCC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCTGTTAAAGCAGCGCAAACTGGGCACTTAGTGCTATCGACCTTGCACACCAATTCTGCCGCCGAAAC
TATTACCCGTTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCAGGCTCTGCCCTCACTGTAAACAAGTGGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCGACTGGC
ACTACCTTATATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGCGTCGGTATCTATGAAGT
GATGCCGTTTAGTCGTCGTCTGGCTCAAGCTCTGGTTGATGGCGCAACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTGACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.989

100

0.62
  pilB Vibrio parahaemolyticus RIMD 2210633

61.279

100

0.613
  pilB Vibrio campbellii strain DS40M4

60.568

100

0.606
  pilB Legionella pneumophila strain ERS1305867

50.184

96.625

0.485
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50.864

92.54

0.471
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366