Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGM67_RS17715 Genome accession   NZ_CP099545
Coordinates   2767316..2768422 (-) Length   368 a.a.
NCBI ID   WP_252689965.1    Uniprot ID   -
Organism   Photobacterium damselae strain XP-11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2762316..2773422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM67_RS17685 (NGM67_17685) - 2762712..2763194 (+) 483 WP_065170682.1 SprT family zinc-dependent metalloprotease -
  NGM67_RS17690 (NGM67_17690) endA 2763325..2764029 (+) 705 WP_005297700.1 deoxyribonuclease I -
  NGM67_RS17695 (NGM67_17695) rsmE 2764161..2764892 (+) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NGM67_RS17700 (NGM67_17700) gshB 2764913..2765860 (+) 948 WP_005297697.1 glutathione synthase -
  NGM67_RS17705 (NGM67_17705) - 2766014..2766577 (+) 564 WP_068945809.1 YqgE/AlgH family protein -
  NGM67_RS17710 (NGM67_17710) ruvX 2766574..2766999 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  NGM67_RS17715 (NGM67_17715) pilU 2767316..2768422 (-) 1107 WP_252689965.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGM67_RS17720 (NGM67_17720) pilT 2768434..2769468 (-) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  NGM67_RS17725 (NGM67_17725) - 2769491..2770192 (+) 702 WP_252689966.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGM67_RS17730 (NGM67_17730) proC 2770208..2771026 (+) 819 WP_252689967.1 pyrroline-5-carboxylate reductase -
  NGM67_RS17735 (NGM67_17735) - 2771038..2771595 (+) 558 WP_005297682.1 YggT family protein -
  NGM67_RS17740 (NGM67_17740) yggU 2771592..2771891 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  NGM67_RS17745 (NGM67_17745) - 2771993..2772586 (+) 594 WP_044174415.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41368.53 Da        Isoelectric Point: 5.5884

>NTDB_id=700574 NGM67_RS17715 WP_252689965.1 2767316..2768422(-) (pilU) [Photobacterium damselae strain XP-11]
MKLQEILNQIVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=700574 NGM67_RS17715 WP_252689965.1 2767316..2768422(-) (pilU) [Photobacterium damselae strain XP-11]
ATGAAACTACAAGAAATTTTAAATCAGATTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGAGCAACGGGGTCGGGTA
AATCGACTTCGATGGCAGCAATGACAGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGTATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.691

93.207

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367