Detailed information NTG0698557

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NFK06_RS23125
• Genome accession: NZ_CP099304
• Coordinates: 4898296..4899093 (+)
• Length: 265 a.a.
• NCBI ID: WP_181251226.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain RHB20-SO-C02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4893296..4904093

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NFK06_RS23090 (NFK06_23065)	gspH	4893437..4893949 (+)	513	WP_014837429.1	type II secretion system minor pseudopilin GspH	-
NFK06_RS23095 (NFK06_23070)	gspI	4893946..4894311 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
NFK06_RS23100 (NFK06_23075)	gspJ	4894308..4894904 (+)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
NFK06_RS23105 (NFK06_23080)	gspK	4894901..4895881 (+)	981	WP_032720565.1	type II secretion system minor pseudopilin GspK	-
NFK06_RS23110 (NFK06_23085)	gspL	4895874..4897067 (+)	1194	WP_181251224.1	type II secretion system protein GspL	-
NFK06_RS23115 (NFK06_23090)	-	4897060..4897545 (+)	486	WP_032694397.1	type II secretion system protein M	-
NFK06_RS23120 (NFK06_23095)	-	4897535..4898293 (+)	759	WP_181251225.1	type II secretion system protein N	-
NFK06_RS23125 (NFK06_23100)	pilD	4898296..4899093 (+)	798	WP_181251226.1	A24 family peptidase	Machinery gene
NFK06_RS23130 (NFK06_23105)	hrpB	4899191..4901620 (-)	2430	WP_181251227.1	ATP-dependent helicase HrpB	-
NFK06_RS23135 (NFK06_23110)	thpR	4901693..4902229 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
NFK06_RS23140 (NFK06_23115)	sfsA	4902229..4902945 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
NFK06_RS23145 (NFK06_23120)	dksA	4903110..4903565 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29200.79 Da
• Isoelectric Point: 8.3358

>NTDB_id=698557 NFK06_RS23125 WP_181251226.1 4898296..4899093(+) (pilD) [Klebsiella michiganensis strain RHB20-SO-C02]
MENIALFPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=698557 NFK06_RS23125 WP_181251226.1 4898296..4899093(+) (pilD) [Klebsiella michiganensis strain RHB20-SO-C02]
ATGGAAAATATCGCGCTGTTCCCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACTGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	47.431	95.472	0.453
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434