Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NE421_RS01630 Genome accession   NZ_CP098791
Coordinates   342695..344407 (-) Length   570 a.a.
NCBI ID   WP_031966873.1    Uniprot ID   A0A505MH52
Organism   Acinetobacter baumannii strain 280820     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337695..349407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE421_RS01595 (NE421_01595) - 337857..338183 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  NE421_RS01600 (NE421_01600) rlmB 338285..339034 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NE421_RS01605 (NE421_01605) - 339070..339984 (+) 915 WP_000075020.1 DMT family transporter -
  NE421_RS01610 (NE421_01610) coaE 339981..340577 (-) 597 WP_000874125.1 dephospho-CoA kinase -
  NE421_RS20585 pilD 340579..341439 (-) 861 WP_031966875.1 A24 family peptidase Machinery gene
  NE421_RS01625 (NE421_01625) pilC 341439..342665 (-) 1227 WP_000279218.1 type II secretion system F family protein Machinery gene
  NE421_RS01630 (NE421_01630) pilB 342695..344407 (-) 1713 WP_031966873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NE421_RS01635 (NE421_01635) tpiA 344698..345492 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  NE421_RS01640 (NE421_01640) secG 345505..345834 (+) 330 WP_000555570.1 preprotein translocase subunit SecG -
  NE421_RS01660 (NE421_01660) rimP 346476..347000 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  NE421_RS01665 (NE421_01665) nusA 347038..348522 (+) 1485 WP_000532248.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63335.72 Da        Isoelectric Point: 5.2391

>NTDB_id=698013 NE421_RS01630 WP_031966873.1 342695..344407(-) (pilB) [Acinetobacter baumannii strain 280820]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEL
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=698013 NE421_RS01630 WP_031966873.1 342695..344407(-) (pilB) [Acinetobacter baumannii strain 280820]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCAAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCACCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGCTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTTTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTCTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTCATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCCACTGCCGAAGACCCAGTAGAAATTAACCTTGAAGGAATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGTAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAACCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A505MH52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.474

100

0.995
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.887

92.982

0.482
  pilB Vibrio campbellii strain DS40M4

51.06

91.053

0.465
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.655

98.596

0.361