Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NDQ71_RS08510 Genome accession   NZ_CP098523
Coordinates   1802018..1803697 (+) Length   559 a.a.
NCBI ID   WP_283710120.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KG3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1797018..1808697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDQ71_RS08495 (NDQ71_08480) pilC 1797416..1798642 (+) 1227 WP_301562580.1 type II secretion system F family protein Machinery gene
  NDQ71_RS08500 (NDQ71_08485) pilD 1800355..1801254 (+) 900 WP_301562581.1 A24 family peptidase Machinery gene
  NDQ71_RS08505 (NDQ71_08490) coaE 1801256..1801885 (+) 630 WP_301562582.1 dephospho-CoA kinase -
  NDQ71_RS08510 (NDQ71_08495) pilB 1802018..1803697 (+) 1680 WP_283710120.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NDQ71_RS08515 (NDQ71_08500) yacG 1803759..1803989 (+) 231 WP_064667149.1 DNA gyrase inhibitor YacG -
  NDQ71_RS08520 (NDQ71_08505) - 1805007..1806239 (-) 1233 WP_301562583.1 NRAMP family divalent metal transporter -
  NDQ71_RS08525 (NDQ71_08510) raiA 1806373..1806729 (-) 357 WP_301562584.1 ribosome-associated translation inhibitor RaiA -
  NDQ71_RS08530 (NDQ71_08515) - 1806913..1807224 (-) 312 WP_192541405.1 pyrimidine/purine nucleoside phosphorylase -
  NDQ71_RS08535 (NDQ71_08520) - 1807544..1808350 (-) 807 WP_192541406.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62348.73 Da        Isoelectric Point: 5.7629

>NTDB_id=696604 NDQ71_RS08510 WP_283710120.1 1802018..1803697(+) (pilB) [Pseudoalteromonas sp. KG3]
MNINSPLLRKFITLGRIDAETVKLKQREYASTAELIAKCSGIDGHELAEQCIDLFRVPYFDLRDFNVELIPKELVKEKLI
KEHHILPLVKKGRKLYIAASDPTDYGAFENFEFSTGLSCEVVVVDYRQLEAKIEQLLDASGSLHLSDEEFKEFSDLDADG
PREQQNNGEDEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRVRFRIDGILHEMASPPNSLATRLSARIKVMARLD
IAEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALQQPQGMILVTGP
TGSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPRAEMTFANALKAFLRQDPDVVMVGEIRDLETAEISI
KAAQTGHLVMSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKAPETLPSEELQRQGFSPEQISELT
LFAPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGLRKAAAGMTSLAEINRVTSF

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=696604 NDQ71_RS08510 WP_283710120.1 1802018..1803697(+) (pilB) [Pseudoalteromonas sp. KG3]
ATGAATATTAATTCACCATTACTTAGAAAGTTTATTACGCTTGGCCGTATTGACGCAGAAACAGTCAAACTCAAACAACG
TGAGTATGCCTCAACAGCTGAGCTGATCGCTAAATGCAGCGGTATTGATGGCCATGAACTTGCCGAGCAATGCATTGATT
TATTCCGAGTTCCCTATTTTGACCTGCGTGATTTTAATGTAGAACTGATCCCAAAAGAGTTAGTTAAAGAAAAACTGATC
AAAGAACATCACATTCTCCCTCTCGTTAAAAAAGGTCGTAAGCTTTATATTGCCGCCTCTGATCCGACTGATTACGGCGC
CTTTGAGAATTTTGAATTTAGTACTGGGCTTTCGTGTGAAGTCGTGGTGGTTGATTATCGTCAGCTCGAAGCAAAAATTG
AGCAACTACTTGATGCCTCAGGCAGCTTGCACTTAAGCGATGAAGAATTTAAAGAATTCAGTGATCTAGATGCCGATGGC
CCACGCGAGCAGCAAAACAACGGTGAAGATGAAAAAGACGATGCGCCAATTATTGTCTACATCAATAAAATTTTGATGGA
TGCAATTAAAAAAGGCGCCTCAGATTTACACTTTGAACCCTATGAGCATAAATACCGCGTACGTTTTCGTATTGATGGTA
TTTTGCATGAAATGGCCAGCCCGCCAAACAGCTTAGCTACGCGTTTATCGGCACGTATAAAAGTAATGGCACGTTTAGAT
ATCGCTGAAAAACGTAAACCACAAGATGGCCGTATCAAGTTAAAAATCACCGAACGTAAAAGCATTGATTTCCGGGTAAG
TACCCTGCCGACCTTATGGGGCGAAAAAATCGTAATGCGTATCCTTGATTCATCAAGTGCAATGTTAGGCATCGATGTAC
TTGGTTATGAACCTGAGCAGAAAAAGCTCTATATGGATGCGCTGCAACAACCACAAGGCATGATCTTAGTTACCGGCCCT
ACGGGCTCTGGTAAAACGGTTTCGCTTTACACGGGTTTGAATATTCTTAACCAGCCGGAACGCAATATTAGTACCGCAGA
AGATCCGGTTGAAATCAACCTCGAAGGGATAAATCAAGTACAAATTAACCCGCGCGCCGAGATGACCTTTGCCAATGCGC
TAAAAGCCTTCTTACGTCAAGATCCTGATGTGGTGATGGTGGGTGAGATCCGAGACCTTGAAACTGCTGAAATTTCAATA
AAAGCGGCGCAAACGGGTCACTTGGTAATGTCTACCTTGCATACCAACTCAGCACCAGAAACCCTAACGCGTTTACTTAA
TATGGGCGTGCCTGCGTATAACGTGGCCAGCTCAATTAGCTTGATCATCGCACAGCGTTTAGCCCGTCGTTTATGCCCAA
AATGTAAAGCACCTGAAACGTTGCCAAGTGAAGAGTTACAACGCCAGGGCTTTAGCCCAGAACAAATAAGCGAACTCACT
TTATTTGCTCCCAAAGGCTGTGACAGCTGCACCGATGGCTATAAAGGCCGGGTAGGTATTTATGAAGTAATGCAAATTAC
CCCTGAAATTGCGCAAATTATTATGCGTGGTGGTAACTCATTAGAAATAGCCGAGGTATCATTGAAGGCTGGTTTTAATA
ATTTACGCTTATCAGGCCTACGTAAAGCCGCCGCCGGTATGACCTCGCTTGCAGAAATAAACCGCGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.165

92.129

0.564
  pilB Acinetobacter baylyi ADP1

59.528

91.055

0.542
  pilB Legionella pneumophila strain ERS1305867

53.235

96.78

0.515
  pilB Vibrio cholerae strain A1552

53.545

95.886

0.513
  pilB Vibrio campbellii strain DS40M4

52.425

95.886

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

53.743

93.202

0.501
  pilF Neisseria gonorrhoeae MS11

49.712

93.202

0.463