Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NCG89_RS05655 Genome accession   NZ_CP098455
Coordinates   1218475..1220193 (+) Length   572 a.a.
NCBI ID   WP_251088793.1    Uniprot ID   -
Organism   Spongiibacter taiwanensis strain SPT1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1207324..1217596 1218475..1220193 flank 879


Gene organization within MGE regions


Location: 1207324..1220193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCG89_RS05600 (NCG89_05600) - 1207324..1208097 (+) 774 WP_251088785.1 Stf0 family sulfotransferase -
  NCG89_RS05605 (NCG89_05605) - 1208104..1208922 (+) 819 WP_251089344.1 polysaccharide pyruvyl transferase family protein -
  NCG89_RS05610 (NCG89_05610) - 1208913..1209821 (-) 909 WP_251088786.1 stealth conserved region 3 domain-containing protein -
  NCG89_RS05615 (NCG89_05615) - 1209832..1210644 (-) 813 WP_251088787.1 glycosyltransferase family 25 protein -
  NCG89_RS05620 (NCG89_05620) - 1211457..1211849 (-) 393 WP_251088788.1 adenylyltransferase/cytidyltransferase family protein -
  NCG89_RS05625 (NCG89_05625) - 1211859..1213013 (-) 1155 WP_251088789.1 CDP-glycerol glycerophosphotransferase family protein -
  NCG89_RS05630 (NCG89_05630) - 1213010..1214722 (-) 1713 WP_251088790.1 ABC transporter ATP-binding protein -
  NCG89_RS05635 (NCG89_05635) - 1214789..1215649 (-) 861 WP_251088791.1 glycosyltransferase family 2 protein -
  NCG89_RS05640 (NCG89_05640) - 1215659..1217560 (-) 1902 WP_251088792.1 hypothetical protein -
  NCG89_RS05645 (NCG89_05645) - 1217723..1218166 (-) 444 WP_285236394.1 pilin -
  NCG89_RS05655 (NCG89_05655) pilB 1218475..1220193 (+) 1719 WP_251088793.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62428.64 Da        Isoelectric Point: 5.0179

>NTDB_id=695600 NCG89_RS05655 WP_251088793.1 1218475..1220193(+) (pilB) [Spongiibacter taiwanensis strain SPT1]
MNSTQPPLNGLPRRLVYDGLLEETEIRKAIDAAQKDKKSLVAYLVSKNLADGIKIAEAAADEFGIPFLDLRAYLPELAPQ
KLVDSKLVRAQHALPLFKRGKRLFLGVSDPTNLAALDQIKFHTGLTTEPIIVEESALNAAIDKYLSAQEESLADSLGDMD
DIGLENLDIEAVDEDGDGDVDLKGADEAPVVKFVNKVLVDAIRGGASDVHFEPYEKIYRVRFRTDGILHEVARPPAALGY
RLAARLKVMSQMDISERRIPQDGRIKMKLSKTRSIDFRVNTLPTLWGEKVVLRILDPSSAQMGIDALGYEEDQKALYMKA
LSQPQGMILVTGPTGSGKTVSLYTGLNILNTPELNISTAEDPVEINLEGINQVHVNPKVGLGFAEALRSFLRQDPDIIMV
GEIRDLETAEIGIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSFNVATSVSLIIAQRLARRLCKECAVPADIPQHTLL
EEGFTPELLDGASIMAPVGCEKCNNGYKGRVGIYEVVRITPEISHIIMENGNSLEIAAKAQEQGFANLRQSALKKCAQGV
TSLEEVNRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=695600 NCG89_RS05655 WP_251088793.1 1218475..1220193(+) (pilB) [Spongiibacter taiwanensis strain SPT1]
ATGAACAGTACCCAACCCCCTCTCAATGGCTTACCCCGACGTTTGGTTTACGATGGCCTTTTAGAAGAGACCGAAATACG
CAAAGCTATCGATGCCGCCCAAAAAGATAAAAAAAGCTTAGTAGCTTACCTGGTCAGCAAGAATCTGGCTGACGGCATCA
AGATTGCCGAGGCTGCCGCCGATGAGTTTGGCATCCCTTTTCTGGATCTGCGGGCGTATCTCCCTGAGCTTGCCCCCCAA
AAATTGGTCGACAGCAAGCTGGTCCGCGCCCAGCACGCCCTCCCCTTGTTTAAACGAGGCAAGCGCCTGTTTCTTGGGGT
TTCCGATCCGACGAATCTGGCCGCCCTTGATCAGATCAAATTCCATACCGGCCTGACCACCGAGCCAATCATTGTCGAAG
AATCTGCGCTGAACGCAGCGATCGACAAATACCTCTCTGCCCAGGAGGAGTCTTTGGCAGACAGTCTGGGCGATATGGAT
GATATCGGCCTTGAGAATCTTGATATCGAAGCCGTAGATGAGGACGGCGATGGCGATGTTGATCTAAAGGGCGCCGACGA
GGCGCCCGTCGTCAAGTTCGTTAACAAGGTATTGGTGGATGCCATTCGCGGCGGCGCATCAGATGTTCACTTTGAGCCCT
ATGAGAAAATCTACCGGGTGCGTTTCCGTACCGACGGTATTCTTCATGAGGTCGCCCGTCCGCCAGCGGCACTAGGCTAC
CGTTTGGCAGCGCGACTAAAAGTCATGTCACAAATGGACATCTCCGAACGACGCATTCCTCAGGATGGTCGCATCAAGAT
GAAACTGTCCAAGACCCGCTCCATCGACTTCCGGGTAAACACCCTGCCCACCTTGTGGGGCGAAAAAGTGGTGCTGCGGA
TTCTGGACCCCAGCTCTGCACAGATGGGTATTGATGCGCTGGGTTATGAGGAAGATCAGAAAGCCCTTTATATGAAGGCC
TTGAGCCAGCCTCAGGGGATGATATTAGTCACGGGCCCGACCGGGTCCGGTAAAACGGTGTCGCTGTACACCGGCCTGAA
CATTCTTAACACGCCGGAGCTGAATATTTCCACCGCTGAAGACCCGGTGGAAATCAACCTCGAAGGCATCAACCAGGTCC
ACGTCAATCCAAAGGTCGGCCTGGGCTTTGCCGAGGCCTTGCGCTCCTTCCTGCGCCAGGACCCCGACATCATTATGGTG
GGGGAGATTCGGGACTTGGAGACCGCAGAAATTGGTATTAAAGCGGCCCAGACCGGGCACTTGGTGTTATCCACCCTGCA
CACCAATAGCGCTGCCGAAACACTAACGCGGCTGTTGAATATGGGTGTGCCATCGTTCAACGTTGCCACCTCAGTCAGCT
TGATTATCGCCCAGCGACTCGCACGACGACTGTGCAAGGAGTGCGCTGTACCAGCGGATATTCCTCAGCACACCTTGCTG
GAAGAGGGATTTACACCGGAGCTATTGGACGGCGCGAGCATTATGGCCCCAGTGGGCTGTGAAAAATGTAACAATGGCTA
TAAGGGGCGGGTGGGCATTTACGAGGTTGTGCGTATTACCCCTGAGATCTCCCACATTATTATGGAAAATGGCAACTCGC
TTGAAATCGCCGCAAAGGCGCAGGAACAGGGCTTTGCCAATTTGCGCCAATCGGCATTAAAAAAATGCGCACAAGGCGTG
ACCAGTCTGGAAGAAGTTAATCGGGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.996

99.825

0.619
  pilB Acinetobacter baylyi ADP1

60.172

100

0.61
  pilB Legionella pneumophila strain ERS1305867

57.143

99.126

0.566
  pilB Vibrio parahaemolyticus RIMD 2210633

51.268

96.503

0.495
  pilB Vibrio campbellii strain DS40M4

49.301

100

0.493
  pilB Vibrio cholerae strain A1552

54.231

90.909

0.493
  pilF Neisseria gonorrhoeae MS11

48.148

99.126

0.477
  pilF Thermus thermophilus HB27

37.368

99.65

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

41.2

87.413

0.36