Detailed information NTG0069523

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVS141_RS12470
• Genome accession: NZ_AP018402
• Coordinates: 2562281..2562895 (-)
• Length: 204 a.a.
• NCBI ID: WP_060562641.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain S141
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2557281..2567895

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVS141_RS12445 (BVS141_24690)	rpsT	2557580..2557846 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVS141_RS12450 (BVS141_24700)	holA	2557886..2558908 (-)	1023	WP_060562639.1	DNA polymerase III subunit delta	-
BVS141_RS19845	-	2558948..2559099 (-)	152	Protein_2387	hypothetical protein	-
BVS141_RS12455 (BVS141_24710)	-	2559140..2559274 (+)	135	WP_003152870.1	YqzM family protein	-
BVS141_RS12460 (BVS141_24720)	comEC	2559293..2561644 (-)	2352	WP_060562640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVS141_RS12465 (BVS141_24730)	-	2561645..2562214 (-)	570	WP_003152868.1	ComE operon protein 2	-
BVS141_RS12470 (BVS141_24740)	comEA	2562281..2562895 (-)	615	WP_060562641.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVS141_RS12475 (BVS141_24750)	comER	2562954..2563775 (+)	822	WP_012118027.1	late competence protein ComER	-
BVS141_RS12480 (BVS141_24760)	-	2563844..2564581 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
BVS141_RS12485 (BVS141_24770)	rsfS	2564578..2564934 (-)	357	WP_007408260.1	ribosome silencing factor	-
BVS141_RS12490 (BVS141_24780)	yqeK	2564952..2565512 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVS141_RS12495 (BVS141_24790)	-	2565502..2566071 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BVS141_RS12500 (BVS141_24800)	yhbY	2566082..2566372 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BVS141_RS12505 (BVS141_24810)	aroE	2566366..2567208 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22050.22 Da
• Isoelectric Point: 6.4803

>NTDB_id=69523 BVS141_RS12470 WP_060562641.1 2562281..2562895(-) (comEA) [Bacillus velezensis strain S141]
MTKAMMTERLLRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLGELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=69523 BVS141_RS12470 WP_060562641.1 2562281..2562895(-) (comEA) [Bacillus velezensis strain S141]
ATGACGAAAGCGATGATGACGGAGCGGCTGCTGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTATAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGGCGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae R6	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	34.906	100	0.363