Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8T91_RS11905 Genome accession   NZ_CP098023
Coordinates   2842133..2843842 (+) Length   569 a.a.
NCBI ID   WP_301414385.1    Uniprot ID   -
Organism   Microbulbifer sp. MI-G     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2837133..2848842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8T91_RS11880 (M8T91_11855) - 2837360..2838529 (+) 1170 WP_301414380.1 DNA polymerase Y family protein -
  M8T91_RS11885 (M8T91_11860) - 2838529..2838726 (+) 198 WP_301414381.1 hypothetical protein -
  M8T91_RS11890 (M8T91_11865) - 2839044..2840012 (-) 969 WP_301414382.1 acyltransferase -
  M8T91_RS11895 (M8T91_11870) - 2840015..2841208 (-) 1194 WP_301414383.1 serine hydrolase -
  M8T91_RS11900 (M8T91_11875) - 2841286..2841753 (-) 468 WP_301414384.1 pilin -
  M8T91_RS11905 (M8T91_11880) pilB 2842133..2843842 (+) 1710 WP_301414385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M8T91_RS11910 (M8T91_11885) pilC 2843994..2845208 (+) 1215 WP_301414386.1 type II secretion system F family protein Machinery gene
  M8T91_RS11915 (M8T91_11890) - 2845229..2846101 (+) 873 WP_301414387.1 A24 family peptidase -
  M8T91_RS11920 (M8T91_11895) coaE 2846177..2846779 (+) 603 WP_301414388.1 dephospho-CoA kinase -
  M8T91_RS11925 (M8T91_11900) - 2846836..2847051 (+) 216 WP_301419083.1 DNA gyrase inhibitor YacG -
  M8T91_RS11930 (M8T91_11905) mutT 2847083..2847496 (-) 414 WP_301419085.1 8-oxo-dGTP diphosphatase MutT -
  M8T91_RS18950 - 2847518..2847640 (-) 123 WP_367317771.1 bifunctional ornithine acetyltransferase/N-acetylglutamate synthase -
  M8T91_RS11935 (M8T91_11910) argJ 2847721..2848788 (-) 1068 Protein_2374 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61974.81 Da        Isoelectric Point: 5.1451

>NTDB_id=693588 M8T91_RS11905 WP_301414385.1 2842133..2843842(+) (pilB) [Microbulbifer sp. MI-G]
MTAPPLSGLAKRLVADQILDATTAAAATQAARRENQTFAQHAVEAKLVKARELAGIAAQAFGTPLFELGSYNFELIPREV
VDEKLIAKHFALPLYQRGNRLFVAVADPTNLAGLDEINFNTGLSTDAVLVEADKLAKAIESYLSHNSDMGAGLEGMDDEA
LDALEVGSDEPAQDDEEPGGDEAPVVRFVNKVLLDAIRSGASDIHFEPYEKTYRVRFRTDGVLHEVAKPPIQLAPRLSAR
LKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAQLGIDALGYEEEQKQLYMDALAQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGVNQVNVSAKVGLDFAEALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKPVTLPHEVLSAEGFA
AVTLPKNTWTLFQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQAREEGFNNLRTSALRKVVMGITSL
EEANRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=693588 M8T91_RS11905 WP_301414385.1 2842133..2843842(+) (pilB) [Microbulbifer sp. MI-G]
ATGACAGCTCCCCCCCTCAGCGGCCTGGCCAAACGCCTGGTGGCAGACCAGATTCTCGACGCAACCACGGCTGCTGCTGC
CACCCAGGCGGCGCGGCGGGAAAACCAGACCTTTGCCCAGCACGCGGTGGAGGCCAAACTGGTCAAGGCCCGCGAGCTTG
CCGGGATTGCCGCCCAAGCCTTTGGCACCCCCTTGTTTGAGCTTGGCAGTTACAACTTCGAGCTGATCCCCAGGGAAGTC
GTCGATGAAAAACTGATCGCCAAACATTTTGCCCTGCCCCTTTACCAGCGGGGCAACCGCCTGTTTGTGGCCGTGGCAGA
CCCCACCAACCTGGCGGGGCTGGATGAAATCAATTTCAACACCGGCCTCAGTACCGACGCGGTGCTGGTGGAGGCGGACA
AGCTGGCGAAAGCCATCGAGAGTTACCTGTCCCATAACAGCGATATGGGTGCGGGCCTGGAGGGCATGGACGACGAAGCG
CTGGACGCCCTGGAGGTGGGCAGCGACGAGCCGGCACAAGATGACGAAGAACCCGGCGGTGACGAGGCCCCTGTTGTACG
CTTTGTGAACAAGGTGCTTCTGGATGCGATACGCAGCGGCGCCTCGGATATTCACTTCGAGCCCTACGAAAAAACCTACC
GGGTCCGCTTCCGCACCGATGGCGTACTGCACGAAGTGGCCAAACCCCCCATCCAGTTGGCACCCCGCCTCTCCGCGCGC
CTGAAGGTGATGTCGAAAATGGATATCTCCGAACGGCGCGTACCCCAAGATGGGCGCATCAAGATGAAGCTGTCAAAAAC
CAAGGCCATCGATTTCCGCGTCAACAGCCTGCCCACCCTGTGGGGGGAAAAGATCGTGCTGCGGATTCTGGACCCCTCTT
CCGCCCAGTTGGGTATCGATGCACTGGGCTATGAAGAGGAGCAAAAACAACTGTATATGGACGCCCTGGCCCAGCCCCAG
GGCATGATCCTGGTGACCGGCCCCACCGGTTCCGGCAAAACCGTGTCCCTCTACACCGGGCTCAATATTCTCAACACCCC
GGAACGCAATATCTCCACCGCGGAAGACCCGGTGGAAATCAACCTGGAGGGGGTCAACCAGGTGAATGTCTCCGCCAAGG
TGGGACTGGACTTTGCCGAGGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGACATCGTGATGGTAGGGGAGATCCGCGAC
CTGGAGACCGCCGAGATTGCCATCAAGGCCGCACAGACCGGGCACCTGGTGTTGTCCACCCTGCACACCAACTCGGCCCC
GGAAACCCTCACGCGCTTGATGAATATGGGTGTGCCCACCTTTAATATCGCCACCTCGGTGAGTGTGATTATCGCCCAGC
GATTGGCACGGCGGCTGTGCAATGAGTGCAAAAAACCCGTCACACTGCCACATGAGGTACTGTCGGCAGAGGGCTTTGCC
GCAGTGACCCTCCCCAAAAACACCTGGACGCTGTTCCAGCCGGTGGGTTGCGAGCACTGCTCCAAAGGCTACAAGGGACG
CGTGGGGGTGTATGAAGTGGTTCGCATTACCGATGGCATCTCCAGAATTATAATGGAGGGCGGCAACTCGATTCAGATTG
CCGACCAGGCCCGCGAGGAGGGCTTCAACAACCTGAGGACCTCGGCTTTGCGCAAAGTGGTCATGGGCATTACCAGCCTG
GAGGAGGCCAACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.471

100

0.617
  pilB Acinetobacter baylyi ADP1

58.844

100

0.591
  pilB Legionella pneumophila strain ERS1305867

54.674

99.649

0.545
  pilB Vibrio cholerae strain A1552

51.773

99.121

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

54.924

92.794

0.51
  pilB Vibrio campbellii strain DS40M4

53.208

93.146

0.496
  pilF Neisseria gonorrhoeae MS11

48.246

100

0.483
  pilB/pilB1 Synechocystis sp. PCC 6803

40

100

0.432