Detailed information NTG0693419

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8561_RS12725
• Genome accession: NZ_CP097895
• Coordinates: 2628277..2628891 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain B1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2623277..2633891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8561_RS12695 (M8561_12695)	rpsT	2623577..2623843 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M8561_RS12700 (M8561_12700)	holA	2623860..2624901 (-)	1042	Protein_2455	DNA polymerase III subunit delta	-
M8561_RS12705 (M8561_12705)	-	2624941..2625095 (-)	155	Protein_2456	hypothetical protein	-
M8561_RS12710 (M8561_12710)	-	2625136..2625270 (+)	135	WP_003152870.1	YqzM family protein	-
M8561_RS12715 (M8561_12715)	comEC	2625289..2627640 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8561_RS12720 (M8561_12720)	-	2627641..2628210 (-)	570	WP_003152868.1	ComE operon protein 2	-
M8561_RS12725 (M8561_12725)	comEA	2628277..2628891 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8561_RS12730 (M8561_12730)	comER	2628950..2629771 (+)	822	WP_014305449.1	late competence protein ComER	-
M8561_RS12735 (M8561_12735)	-	2629840..2630577 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
M8561_RS12740 (M8561_12740)	rsfS	2630574..2630930 (-)	357	WP_003152864.1	ribosome silencing factor	-
M8561_RS12745 (M8561_12745)	yqeK	2630948..2631508 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8561_RS12750 (M8561_12750)	-	2631498..2632067 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
M8561_RS12755 (M8561_12755)	yhbY	2632078..2632368 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
M8561_RS12760 (M8561_12760)	aroE	2632362..2633204 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=693419 M8561_RS12725 WP_041482377.1 2628277..2628891(-) (comEA) [Bacillus velezensis strain B1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=693419 M8561_RS12725 WP_041482377.1 2628277..2628891(-) (comEA) [Bacillus velezensis strain B1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363