Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9415_RS03840 Genome accession   NZ_CP097790
Coordinates   802832..804217 (+) Length   461 a.a.
NCBI ID   WP_046333442.1    Uniprot ID   -
Organism   Pasteurella multocida strain 31971     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797832..809217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9415_RS03810 (M9415_03810) - 798023..798874 (-) 852 WP_005751072.1 thymidylate synthase -
  M9415_RS03815 (M9415_03815) lgt 798880..799683 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M9415_RS03820 (M9415_03820) - 799693..800487 (-) 795 WP_046333443.1 sulfite exporter TauE/SafE family protein -
  M9415_RS03825 (M9415_03825) rppH 800490..801086 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9415_RS03830 (M9415_03830) ampD 801672..802238 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9415_RS03835 (M9415_03835) pilA 802398..802832 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9415_RS03840 (M9415_03840) pilB 802832..804217 (+) 1386 WP_046333442.1 GspE/PulE family protein Machinery gene
  M9415_RS03845 (M9415_03845) - 804217..805428 (+) 1212 WP_046333441.1 type II secretion system F family protein -
  M9415_RS03850 (M9415_03850) - 805428..806150 (+) 723 WP_046333440.1 A24 family peptidase -
  M9415_RS03855 (M9415_03855) coaE 806165..806785 (+) 621 WP_046333439.1 dephospho-CoA kinase -
  M9415_RS03860 (M9415_03860) yacG 806796..806999 (+) 204 WP_046333438.1 DNA gyrase inhibitor YacG -
  M9415_RS03865 (M9415_03865) - 806999..807268 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9415_RS03870 (M9415_03870) - 807717..809090 (+) 1374 WP_046333437.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52033.67 Da        Isoelectric Point: 6.2343

>NTDB_id=692490 M9415_RS03840 WP_046333442.1 802832..804217(+) (pilB) [Pasteurella multocida strain 31971]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692490 M9415_RS03840 WP_046333442.1 802832..804217(+) (pilB) [Pasteurella multocida strain 31971]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.15

98.482

0.573
  pilB Acinetobacter baumannii D1279779

40.202

100

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.419
  pilB Vibrio cholerae strain A1552

39.22

100

0.414
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.412
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401