Detailed information NTG0692378

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MF619_RS12460
• Genome accession: NZ_CP097784
• Coordinates: 2504838..2505452 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain V 3.14
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2499838..2510452

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MF619_RS12430 (MF619_002479)	rpsT	2500138..2500404 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MF619_RS12435 (MF619_002480)	holA	2500421..2501462 (-)	1042	Protein_2377	DNA polymerase III subunit delta	-
MF619_RS12440 (MF619_002481)	-	2501502..2501656 (-)	155	Protein_2378	hypothetical protein	-
MF619_RS12445 (MF619_002482)	-	2501697..2501831 (+)	135	WP_003152870.1	YqzM family protein	-
MF619_RS12450 (MF619_002483)	comEC	2501850..2504201 (-)	2352	WP_025852986.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MF619_RS12455 (MF619_002484)	-	2504202..2504771 (-)	570	WP_003152868.1	ComE operon protein 2	-
MF619_RS12460 (MF619_002485)	comEA	2504838..2505452 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
MF619_RS12465 (MF619_002486)	comER	2505511..2506332 (+)	822	WP_014305449.1	late competence protein ComER	-
MF619_RS12470 (MF619_002487)	-	2506401..2507138 (-)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
MF619_RS12475 (MF619_002488)	rsfS	2507135..2507491 (-)	357	WP_003152864.1	ribosome silencing factor	-
MF619_RS12480 (MF619_002489)	yqeK	2507509..2508069 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MF619_RS12485 (MF619_002490)	-	2508059..2508628 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
MF619_RS12490 (MF619_002491)	yhbY	2508639..2508929 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MF619_RS12495 (MF619_002492)	aroE	2508923..2509765 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=692378 MF619_RS12460 WP_003152867.1 2504838..2505452(-) (comEA) [Bacillus velezensis strain V 3.14]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=692378 MF619_RS12460 WP_003152867.1 2504838..2505452(-) (comEA) [Bacillus velezensis strain V 3.14]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363