Detailed information NTG0692299

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MF621_RS11830
• Genome accession: NZ_CP097779
• Coordinates: 2434552..2435166 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain R 4.6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2429552..2440166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MF621_RS11800 (MF621_002351)	rpsT	2429852..2430118 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MF621_RS11805 (MF621_002352)	holA	2430135..2431176 (-)	1042	Protein_2307	DNA polymerase III subunit delta	-
MF621_RS11810 (MF621_002353)	-	2431216..2431370 (-)	155	Protein_2308	hypothetical protein	-
MF621_RS11815 (MF621_002354)	-	2431411..2431545 (+)	135	WP_003152870.1	YqzM family protein	-
MF621_RS11820 (MF621_002355)	comEC	2431564..2433915 (-)	2352	WP_025852986.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MF621_RS11825 (MF621_002356)	-	2433916..2434485 (-)	570	WP_003152868.1	ComE operon protein 2	-
MF621_RS11830 (MF621_002357)	comEA	2434552..2435166 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
MF621_RS11835 (MF621_002358)	comER	2435225..2436046 (+)	822	WP_014305449.1	late competence protein ComER	-
MF621_RS11840 (MF621_002359)	-	2436115..2436852 (-)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
MF621_RS11845 (MF621_002360)	rsfS	2436849..2437205 (-)	357	WP_003152864.1	ribosome silencing factor	-
MF621_RS11850 (MF621_002361)	yqeK	2437223..2437783 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MF621_RS11855 (MF621_002362)	-	2437773..2438342 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
MF621_RS11860 (MF621_002363)	yhbY	2438353..2438643 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MF621_RS11865 (MF621_002364)	aroE	2438637..2439479 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=692299 MF621_RS11830 WP_003152867.1 2434552..2435166(-) (comEA) [Bacillus velezensis strain R 4.6]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=692299 MF621_RS11830 WP_003152867.1 2434552..2435166(-) (comEA) [Bacillus velezensis strain R 4.6]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363