NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M8855_RS05735	Genome accession	NZ_CP097620
Coordinates	1246410..1246844 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain P2192
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1241410..1251844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8855_RS05705 (M8855_05705)	-	1241974..1242243 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
M8855_RS05710 (M8855_05710)	yacG	1242243..1242446 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
M8855_RS05715 (M8855_05715)	coaE	1242457..1243077 (-)	621	WP_249983977.1	dephospho-CoA kinase	-
M8855_RS05720 (M8855_05720)	-	1243092..1243814 (-)	723	WP_046333440.1	A24 family peptidase	-
M8855_RS05725 (M8855_05725)	-	1243814..1245025 (-)	1212	WP_005753354.1	type II secretion system F family protein	-
M8855_RS05730 (M8855_05730)	pilB	1245025..1246410 (-)	1386	WP_064968700.1	GspE/PulE family protein	Machinery gene
M8855_RS05735 (M8855_05735)	pilA	1246410..1246844 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
M8855_RS05740 (M8855_05740)	ampD	1247004..1247570 (+)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
M8855_RS05745 (M8855_05745)	rppH	1248156..1248752 (+)	597	WP_249983978.1	RNA pyrophosphohydrolase	-
M8855_RS05750 (M8855_05750)	-	1248755..1249549 (+)	795	WP_170355806.1	sulfite exporter TauE/SafE family protein	-
M8855_RS05755 (M8855_05755)	lgt	1249559..1250362 (+)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
M8855_RS05760 (M8855_05760)	-	1250368..1251219 (+)	852	WP_005751072.1	thymidylate synthase	-
M8855_RS05765 (M8855_05765)	tadA	1251234..1251734 (+)	501	WP_163884068.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=691299 M8855_RS05735 WP_005741096.1 1246410..1246844(-) (pilA) [Pasteurella multocida strain P2192]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=691299 M8855_RS05735 WP_005741096.1 1246410..1246844(-) (pilA) [Pasteurella multocida strain P2192]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0691299