Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8854_RS06235 Genome accession   NZ_CP097618
Coordinates   1320251..1321636 (-) Length   461 a.a.
NCBI ID   WP_064964977.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2095     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1315251..1326636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8854_RS06205 (M8854_06205) - 1315378..1316751 (-) 1374 WP_005722571.1 sodium:alanine symporter family protein -
  M8854_RS06210 (M8854_06210) - 1317200..1317469 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M8854_RS06215 (M8854_06215) yacG 1317469..1317672 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M8854_RS06220 (M8854_06220) coaE 1317683..1318303 (-) 621 WP_059245780.1 dephospho-CoA kinase -
  M8854_RS06225 (M8854_06225) - 1318318..1319040 (-) 723 WP_059245779.1 A24 family peptidase -
  M8854_RS06230 (M8854_06230) - 1319040..1320251 (-) 1212 WP_059245778.1 type II secretion system F family protein -
  M8854_RS06235 (M8854_06235) pilB 1320251..1321636 (-) 1386 WP_064964977.1 GspE/PulE family protein Machinery gene
  M8854_RS06240 (M8854_06240) pilA 1321636..1322070 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M8854_RS06245 (M8854_06245) ampD 1322230..1322796 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8854_RS06250 (M8854_06250) rppH 1323382..1323978 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M8854_RS06255 (M8854_06255) - 1323981..1324775 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M8854_RS06260 (M8854_06260) lgt 1324785..1325588 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M8854_RS06265 (M8854_06265) - 1325594..1326445 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52129.82 Da        Isoelectric Point: 6.2343

>NTDB_id=691277 M8854_RS06235 WP_064964977.1 1320251..1321636(-) (pilB) [Pasteurella multocida strain P2095]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLYPPKLQVEENIPMVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=691277 M8854_RS06235 WP_064964977.1 1320251..1321636(-) (pilB) [Pasteurella multocida strain P2095]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTACCCACCAAAATTACAAGTGGAAGAAAACATACCCATGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.15

98.482

0.573
  pilB Acinetobacter baumannii D1279779

39.877

100

0.423
  pilB Acinetobacter baylyi ADP1

38.277

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.414
  pilB Vibrio cholerae strain A1552

38.889

100

0.41
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395
  pilF Thermus thermophilus HB27

38.426

93.709

0.36