Detailed information NTG0690783

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8962_RS10045
• Genome accession: NZ_CP097591
• Coordinates: 2085364..2085978 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain UA0188
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2080364..2090978

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8962_RS10015	rpsT	2080667..2080933 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M8962_RS10020	holA	2080950..2081991 (-)	1042	Protein_1972	DNA polymerase III subunit delta	-
M8962_RS10025	-	2082031..2082182 (-)	152	Protein_1973	hypothetical protein	-
M8962_RS10030	-	2082223..2082357 (+)	135	WP_003152870.1	YqzM family protein	-
M8962_RS10035	comEC	2082376..2084727 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8962_RS10040	-	2084728..2085297 (-)	570	WP_003152868.1	ComE operon protein 2	-
M8962_RS10045	comEA	2085364..2085978 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8962_RS10050	comER	2086037..2086858 (+)	822	WP_012118027.1	late competence protein ComER	-
M8962_RS10055	-	2086927..2087664 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
M8962_RS10060	rsfS	2087661..2088017 (-)	357	WP_007408260.1	ribosome silencing factor	-
M8962_RS10065	yqeK	2088034..2088594 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8962_RS10070	-	2088584..2089153 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
M8962_RS10075	yhbY	2089164..2089454 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
M8962_RS10080	aroE	2089448..2090290 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=690783 M8962_RS10045 WP_012118026.1 2085364..2085978(-) (comEA) [Bacillus velezensis strain UA0188]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=690783 M8962_RS10045 WP_012118026.1 2085364..2085978(-) (comEA) [Bacillus velezensis strain UA0188]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363