Detailed information NTG0690644

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8966_RS13600
• Genome accession: NZ_CP097589
• Coordinates: 2796495..2797109 (+)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain UA0311
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2791495..2802109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8966_RS13565	aroE	2792183..2793025 (+)	843	WP_012118031.1	shikimate dehydrogenase	-
M8966_RS13570	yhbY	2793019..2793309 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
M8966_RS13575	-	2793320..2793889 (+)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
M8966_RS13580	yqeK	2793879..2794439 (+)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8966_RS13585	rsfS	2794456..2794812 (+)	357	WP_007408260.1	ribosome silencing factor	-
M8966_RS13590	-	2794809..2795546 (+)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
M8966_RS13595	comER	2795615..2796436 (-)	822	WP_012118027.1	late competence protein ComER	-
M8966_RS13600	comEA	2796495..2797109 (+)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8966_RS13605	-	2797176..2797745 (+)	570	WP_003152868.1	ComE operon protein 2	-
M8966_RS13610	comEC	2797746..2800097 (+)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8966_RS13615	-	2800116..2800250 (-)	135	WP_003152870.1	YqzM family protein	-
M8966_RS13620	-	2800291..2800442 (+)	152	Protein_2685	hypothetical protein	-
M8966_RS13625	holA	2800482..2801523 (+)	1042	Protein_2686	DNA polymerase III subunit delta	-
M8966_RS13630	rpsT	2801540..2801806 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=690644 M8966_RS13600 WP_012118026.1 2796495..2797109(+) (comEA) [Bacillus velezensis strain UA0311]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=690644 M8966_RS13600 WP_012118026.1 2796495..2797109(+) (comEA) [Bacillus velezensis strain UA0311]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363