Detailed information NTG0690555

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8961_RS06960
• Genome accession: NZ_CP097588
• Coordinates: 1470853..1471467 (+)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain UA0323
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1465853..1476467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8961_RS06925	aroE	1466541..1467383 (+)	843	WP_012118031.1	shikimate dehydrogenase	-
M8961_RS06930	yhbY	1467377..1467667 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
M8961_RS06935	-	1467678..1468247 (+)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
M8961_RS06940	yqeK	1468237..1468797 (+)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8961_RS06945	rsfS	1468814..1469170 (+)	357	WP_007408260.1	ribosome silencing factor	-
M8961_RS06950	-	1469167..1469904 (+)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
M8961_RS06955	comER	1469973..1470794 (-)	822	WP_012118027.1	late competence protein ComER	-
M8961_RS06960	comEA	1470853..1471467 (+)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8961_RS06965	-	1471534..1472103 (+)	570	WP_003152868.1	ComE operon protein 2	-
M8961_RS06970	comEC	1472104..1474455 (+)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8961_RS06975	-	1474474..1474608 (-)	135	WP_003152870.1	YqzM family protein	-
M8961_RS06980	-	1474649..1474800 (+)	152	Protein_1376	hypothetical protein	-
M8961_RS06985	holA	1474840..1475881 (+)	1042	Protein_1377	DNA polymerase III subunit delta	-
M8961_RS06990	rpsT	1475898..1476164 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=690555 M8961_RS06960 WP_012118026.1 1470853..1471467(+) (comEA) [Bacillus velezensis strain UA0323]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=690555 M8961_RS06960 WP_012118026.1 1470853..1471467(+) (comEA) [Bacillus velezensis strain UA0323]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363