Detailed information NTG0690460

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8957_RS02505
• Genome accession: NZ_CP097587
• Coordinates: 499794..500408 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain UA0276
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 494794..505408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8957_RS02475	rpsT	495097..495363 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M8957_RS02480	holA	495380..496421 (-)	1042	Protein_483	DNA polymerase III subunit delta	-
M8957_RS02485	-	496461..496612 (-)	152	Protein_484	hypothetical protein	-
M8957_RS02490	-	496653..496787 (+)	135	WP_003152870.1	YqzM family protein	-
M8957_RS02495	comEC	496806..499157 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8957_RS02500	-	499158..499727 (-)	570	WP_003152868.1	ComE operon protein 2	-
M8957_RS02505	comEA	499794..500408 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8957_RS02510	comER	500467..501288 (+)	822	WP_012118027.1	late competence protein ComER	-
M8957_RS02515	-	501357..502094 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
M8957_RS02520	rsfS	502091..502447 (-)	357	WP_007408260.1	ribosome silencing factor	-
M8957_RS02525	yqeK	502464..503024 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8957_RS02530	-	503014..503583 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
M8957_RS02535	yhbY	503594..503884 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
M8957_RS02540	aroE	503878..504720 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=690460 M8957_RS02505 WP_012118026.1 499794..500408(-) (comEA) [Bacillus velezensis strain UA0276]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=690460 M8957_RS02505 WP_012118026.1 499794..500408(-) (comEA) [Bacillus velezensis strain UA0276]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363