Detailed information NTG0690405

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M8964_RS07440
• Genome accession: NZ_CP097585
• Coordinates: 1448360..1448974 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain UA1365
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1443360..1453974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8964_RS07410	rpsT	1443663..1443929 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M8964_RS07415	holA	1443946..1444987 (-)	1042	Protein_1459	DNA polymerase III subunit delta	-
M8964_RS07420	-	1445027..1445178 (-)	152	Protein_1460	hypothetical protein	-
M8964_RS07425	-	1445219..1445353 (+)	135	WP_003152870.1	YqzM family protein	-
M8964_RS07430	comEC	1445372..1447723 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8964_RS07435	-	1447724..1448293 (-)	570	WP_003152868.1	ComE operon protein 2	-
M8964_RS07440	comEA	1448360..1448974 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8964_RS07445	comER	1449033..1449854 (+)	822	WP_012118027.1	late competence protein ComER	-
M8964_RS07450	-	1449923..1450660 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
M8964_RS07455	rsfS	1450657..1451013 (-)	357	WP_007408260.1	ribosome silencing factor	-
M8964_RS07460	yqeK	1451030..1451590 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8964_RS07465	-	1451580..1452149 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
M8964_RS07470	yhbY	1452160..1452450 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
M8964_RS07475	aroE	1452444..1453286 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=690405 M8964_RS07440 WP_012118026.1 1448360..1448974(-) (comEA) [Bacillus velezensis strain UA1365]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=690405 M8964_RS07440 WP_012118026.1 1448360..1448974(-) (comEA) [Bacillus velezensis strain UA1365]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363