Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M8G39_RS29860 Genome accession   NZ_CP097383
Coordinates   6335337..6337469 (-) Length   710 a.a.
NCBI ID   WP_023100018.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain L00-a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6330337..6342469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8G39_RS29845 (M8G39_29845) - 6331989..6333644 (-) 1656 WP_003141778.1 AAA family ATPase -
  M8G39_RS29850 (M8G39_29850) aroB 6333651..6334757 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  M8G39_RS29855 (M8G39_29855) aroK 6334807..6335325 (-) 519 WP_003095833.1 shikimate kinase AroK -
  M8G39_RS29860 (M8G39_29860) pilQ 6335337..6337469 (-) 2133 WP_023100018.1 type 4a pilus secretin PilQ Machinery gene
  M8G39_RS29865 (M8G39_29865) pilP 6337523..6338047 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  M8G39_RS29870 (M8G39_29870) pilO 6338044..6338667 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  M8G39_RS29875 (M8G39_29875) pilN 6338664..6339260 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  M8G39_RS29880 (M8G39_29880) pilM 6339260..6340324 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77345.79 Da        Isoelectric Point: 5.3155

>NTDB_id=689377 M8G39_RS29860 WP_023100018.1 6335337..6337469(-) (pilQ) [Pseudomonas aeruginosa strain L00-a]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVRASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SSGEEKENSRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNS
YGKNGNMGIKDKEGYNCGPFQGQCTFPTRDNSPTPFVDMGAKDASSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKV
VTSDKETAKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNA
KILVNDGETIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=689377 M8G39_RS29860 WP_023100018.1 6335337..6337469(-) (pilQ) [Pseudomonas aeruginosa strain L00-a]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAGGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAACCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAATCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATTGCCAAGCTGTTCCAGTCGGTTACT
AGTAGTGGCGAAGAGAAAGAAAATTCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCC
GCAGGAGCGGCTGGACGAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTA
TCGTCGAGGCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGTACGTTCCC
GACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTAGTTCCGGTATCGGTATTGGCTTTATCA
CCGATAACATCATTCTTGACTTGCAATTATCGGCCATGGAGAAAACCGGGAACGGCGAAGTGGTTTCGCAACCCAAGGTC
GTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATCCCATATCAGGAGGCCAGTTCCAGTGGTGCGAC
CAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTGACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGG
TGAAGGTGACCAAGGATGCTCCCGACTTCCAGAATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCC
AAGATTCTGGTCAACGATGGTGAAACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGT
ACCCTTCCTCGGTGACCTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGATACGAAAAACGAACTTCTGG
TTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(545-701)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

94.678

100

0.952
  comQ Acinetobacter baylyi ADP1

42.368

98.732

0.418
  pilQ Acinetobacter baumannii strain A118

40.621

99.859

0.406
  pilQ Acinetobacter baumannii D1279779

40.621

99.859

0.406
  pilQ Legionella pneumophila strain Lp02

38.248

96.479

0.369
  pilQ Legionella pneumophila strain ERS1305867

38.248

96.479

0.369