Detailed information NTG0688258

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M4X66_RS10890
• Genome accession: NZ_CP097286
• Coordinates: 2472614..2473780 (-)
• Length: 388 a.a.
• NCBI ID: WP_004879571.1
• Uniprot ID: A0AA46VZ15
• Organism: Pseudomonas viridiflava strain JACO-C-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2467614..2478780

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M4X66_RS10875	-	2468777..2470189 (+)	1413	WP_122422159.1	sigma 54-interacting transcriptional regulator	-
M4X66_RS10880	-	2470441..2471832 (+)	1392	WP_057408916.1	GntP family permease	-
M4X66_RS10885	-	2471843..2472601 (+)	759	WP_004879569.1	3-hydroxybutyrate dehydrogenase	-
M4X66_RS10890	pilU	2472614..2473780 (-)	1167	WP_004879571.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M4X66_RS10895	-	2473892..2474167 (+)	276	WP_122668261.1	peptidylprolyl isomerase	-
M4X66_RS10900	pgm	2474222..2475868 (-)	1647	WP_069144155.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
M4X66_RS10905	-	2475976..2478231 (-)	2256	WP_249584695.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42759.10 Da
• Isoelectric Point: 6.6698

>NTDB_id=688258 M4X66_RS10890 WP_004879571.1 2472614..2473780(-) (pilU) [Pseudomonas viridiflava strain JACO-C-5]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGTETLKPGEVAKIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLYLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNQLNELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKHADSQNNVRLRLKLHGENGVNTLTTAPAAPTGAAKPSMVEWGLVDDEPPSQS

Nucleotide

• Length: 1167 bp

>NTDB_id=688258 M4X66_RS10890 WP_004879571.1 2472614..2473780(-) (pilU) [Pseudomonas viridiflava strain JACO-C-5]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCCACCGGAGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCACTGGGCACCGAAACCCTCAAGCCGGGCGAAGTGGCGAAGATCGCTCAGGGCC
TGATGGACGAAGAGCAGAAGCTGGAATTCCTGCGCGAACTGGAAATGAACCTCGCCGTTTCACTGGCGGGCATCGGGCGT
TTCCGGATCAACATCTTCATGCAGCGCAACGAAGTATCGATTGTCGCGCGTAACATCAAGCTGGATATTCCGCGCTTCGA
AGACCTGTATCTGCCGCCGGTTCTGCTCGATGTGATCATGGAAAAGCACGGCCTGGTGCTGTTCGTCGGTGCCACCGGTT
CGGGTAAGTCCACCTCATTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAATTCATTCATCGACACAAGAAATCCATCGTCAATCAGCGGGAAGTAGGCGTCGATACCCGCAGCTTCAGGGC
GGCGCTGAAAAACACCCTGCGCCAAGCGCCGGATGTGATTCTGATCGGCGAGATCCGCGACCGTGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGGCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAACGACGTCCGCAATTGCTGCATGACCTGGGTAACAACCTCAAGGCGTTCGTGTCGCAGCG
TCTGGTCAAAACTCCGGATGGCAAGCGCCGTGCTGCGGTTGAGGTGATGATGGGCACGCCGACCATTCGTGATCTGATCC
AGCGTAACCAGTTGAACGAGCTCAAAGGGATCATGGAGAAGTCCGGCAGCCTCGGCATGCAGACCTTCGACACTGCGTTG
TTCAACCTTGCGGTGGAGGGTGCGATCAGCGAAGAGGAAGCGCTGAAGCATGCGGATTCGCAGAACAACGTGCGCCTGCG
CCTCAAGTTGCACGGCGAAAACGGGGTGAACACCCTGACGACAGCGCCTGCTGCGCCGACCGGCGCGGCCAAGCCGAGCA
TGGTTGAGTGGGGGCTGGTCGACGACGAGCCCCCGTCCCAGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.065	94.845	0.541
pilU	Acinetobacter baylyi ADP1	51.831	91.495	0.474
pilU	Vibrio cholerae strain A1552	51.143	90.206	0.461
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.51	94.588	0.374
pilT	Pseudomonas aeruginosa PAK	41.108	88.402	0.363
pilT	Pseudomonas stutzeri DSM 10701	40.816	88.402	0.361