Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5C96_RS23020 Genome accession   NZ_CP097269
Coordinates   4918393..4920123 (+) Length   576 a.a.
NCBI ID   WP_272565513.1    Uniprot ID   A0AAN5AYR4
Organism   Acidovorax sp. GBBC 1281     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4913393..4925123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C96_RS22990 (M5C96_22985) proB 4913998..4915137 (-) 1140 WP_272565509.1 glutamate 5-kinase -
  M5C96_RS22995 (M5C96_22990) obgE 4915219..4916289 (-) 1071 WP_272565511.1 GTPase ObgE -
  M5C96_RS23000 (M5C96_22995) rpmA 4916413..4916670 (-) 258 WP_272548923.1 50S ribosomal protein L27 -
  M5C96_RS23005 (M5C96_23000) rplU 4916685..4916996 (-) 312 WP_272565512.1 50S ribosomal protein L21 -
  M5C96_RS23010 (M5C96_23005) - 4917253..4918182 (+) 930 WP_272569825.1 polyprenyl synthetase family protein -
  M5C96_RS23020 (M5C96_23015) pilB 4918393..4920123 (+) 1731 WP_272565513.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M5C96_RS23025 (M5C96_23020) pilC 4920144..4921361 (+) 1218 WP_272565514.1 type II secretion system F family protein Machinery gene
  M5C96_RS23030 (M5C96_23025) - 4921361..4922245 (+) 885 WP_272565516.1 A24 family peptidase -
  M5C96_RS23035 (M5C96_23030) coaE 4922250..4922870 (+) 621 WP_272565518.1 dephospho-CoA kinase -
  M5C96_RS23040 (M5C96_23035) zapD 4922935..4923690 (+) 756 WP_272565520.1 cell division protein ZapD -
  M5C96_RS23045 (M5C96_23040) - 4923704..4923952 (+) 249 WP_272565521.1 DNA gyrase inhibitor YacG -
  M5C96_RS23050 (M5C96_23045) - 4923993..4924448 (-) 456 WP_272565522.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62776.27 Da        Isoelectric Point: 5.6166

>NTDB_id=688243 M5C96_RS23020 WP_272565513.1 4918393..4920123(+) (pilB) [Acidovorax sp. GBBC 1281]
MAVDTAPKEGAAIALPGLGRALMSAGKLSQKSAEDIFRKSQVGKTSFIAELTGSGAVSSADLAHTVSAVFGAPLLDLEAI
DPLRIPKDLLDPKICIAYRVVALSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLIKLIEANTKSVSESME
SLSGGGDFEFDDVKVEEAPENSDSANDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIA
IKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKERL
MHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPDI
IMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKAPADIPHE
TLLEAGYKEDEIDGTWVTYRPVGCSACNNGYKGRLGIYQVMPISEEIQRIILRDGSALEIAKQAKAEGVRSLREAGLYKV
KMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=688243 M5C96_RS23020 WP_272565513.1 4918393..4920123(+) (pilB) [Acidovorax sp. GBBC 1281]
ATGGCCGTTGATACCGCACCCAAAGAAGGCGCCGCAATTGCCCTGCCAGGTCTAGGCCGGGCATTGATGTCAGCCGGCAA
GCTGAGTCAAAAATCAGCCGAAGATATCTTTCGCAAGTCACAAGTCGGCAAAACAAGCTTCATTGCCGAGTTGACCGGGT
CGGGGGCTGTTTCCTCTGCCGATCTGGCGCACACGGTTTCTGCGGTATTTGGTGCGCCCTTGCTGGATCTGGAAGCCATC
GATCCTCTTCGAATTCCCAAAGATCTCCTGGATCCGAAGATCTGCATCGCCTATCGTGTTGTGGCCCTGAGCAAGCGCAA
CAACCGGCTGATCGTGGCCACCGCAGACCCTACCGACCAGGAGGCAGCGGAAAAGATCAAATTCACGACCCAGATGGGAG
TGGACTGGATCATTGCGGAATACGACAAGCTCATCAAGCTGATCGAGGCCAATACCAAGTCGGTCAGCGAGTCGATGGAA
AGCCTGTCGGGCGGGGGAGATTTCGAATTCGACGATGTCAAGGTCGAAGAAGCCCCCGAAAACTCGGACAGCGCCAACGA
CGTTGAAGATGCGCCGATCGTCAAGTTCCTGCACAAGATGCTTTTGGACGCGTTCAACATGCGTGCGTCCGATTTGCATT
TCGAGCCTTACGAACACCAGTACCGGGTGCGTTTTCGGATCGATGGCGAATTGCGTGAAATCGCTTCGCCCCCCATCGCC
ATCAAGGACAAGCTGGCCTCCCGGATCAAGGTCATTTCCCGACTGGATATTTCGGAAAAGCGCGTCCCACAAGATGGCCG
CATGAAGCTCAAAGTCGGCCCGGATCGGGTCATCGACTTTCGGGTGAGCACGCTGCCAACGCTCTTTGGCGAAAAGATCG
TGATCCGGATTTTGGATCCCAGCAGCGCCAAGCTGGGCATCGATGCGCTGGGTTACGAGCCGGAAGAAAAGGAACGGCTG
ATGCATGCCATCGGCCGGCCTTACGGCATGATTTTGGTGACCGGGCCAACGGGTTCAGGGAAAACCGTTTCCTTGTACAC
CTGCCTGAATTTGCTGAATAAAGCAGGCGTCAACATCGCGACCGCGGAGGACCCCTCGGAAATCAATTTGCCTGGTGTCA
ACCAAGTGAATGTCAATGAGAAAGCGGGGCTGACCTTTGCCACCGCCCTCAAGTCGTTTCTGCGTCAAGATCCTGACATC
ATCATGGTCGGCGAAATTCGCGACCTTGAAACGGCGGATATTTCCATCAAGGCGGCACAGACGGGCCACTTGGTGCTGTC
CACCCTCCACACCAACGATGCTCCGACCACGCTCACCCGGATGCGCAATATGGGAATTGCGCCATTCAACATTGCGTCCA
GCGTCATTCTGATAACGGCCCAGCGCCTGGCTCGCCGTTTGTGCCCGGTCTGCAAAGCACCCGCCGATATTCCACATGAA
ACCCTGCTCGAAGCAGGCTATAAAGAAGACGAAATCGATGGCACCTGGGTGACCTATCGTCCTGTCGGTTGTTCCGCTTG
CAACAATGGCTACAAGGGGCGGCTGGGCATCTATCAGGTCATGCCGATTTCCGAAGAAATTCAACGCATTATTTTGCGCG
ATGGAAGTGCCTTGGAAATCGCCAAGCAGGCCAAGGCGGAGGGAGTTCGCTCCTTGCGCGAAGCGGGCCTTTATAAAGTC
AAGATGGGGCTGACCTCTCTCGAAGAGGTGCTGGCCGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.938

100

0.547
  pilB Acinetobacter baumannii D1279779

54.059

100

0.543
  pilF Neisseria gonorrhoeae MS11

52.482

97.917

0.514
  pilB Legionella pneumophila strain ERS1305867

49.912

98.09

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

45.552

97.569

0.444
  pilB Vibrio campbellii strain DS40M4

44.938

97.743

0.439
  pilB Vibrio cholerae strain A1552

47.389

89.757

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.547

99.653

0.394
  pilF Thermus thermophilus HB27

38.908

98.611

0.384