Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5C98_RS18565 Genome accession   NZ_CP097267
Coordinates   4068082..4069812 (+) Length   576 a.a.
NCBI ID   WP_272553337.1    Uniprot ID   -
Organism   Acidovorax sp. NCPPB 3576     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4063082..4074812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C98_RS18535 (M5C98_18535) proB 4063688..4064827 (-) 1140 WP_272553335.1 glutamate 5-kinase -
  M5C98_RS18540 (M5C98_18540) obgE 4064910..4065980 (-) 1071 WP_272548922.1 GTPase ObgE -
  M5C98_RS18545 (M5C98_18545) rpmA 4066104..4066361 (-) 258 WP_272548923.1 50S ribosomal protein L27 -
  M5C98_RS18550 (M5C98_18550) rplU 4066376..4066687 (-) 312 WP_092741307.1 50S ribosomal protein L21 -
  M5C98_RS18555 (M5C98_18555) - 4066944..4067873 (+) 930 WP_272553336.1 polyprenyl synthetase family protein -
  M5C98_RS18565 (M5C98_18565) pilB 4068082..4069812 (+) 1731 WP_272553337.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M5C98_RS18570 (M5C98_18570) pilC 4069833..4071050 (+) 1218 WP_272548924.1 type II secretion system F family protein Machinery gene
  M5C98_RS18575 (M5C98_18575) - 4071050..4071934 (+) 885 WP_272548925.1 A24 family peptidase -
  M5C98_RS18580 (M5C98_18580) coaE 4071942..4072562 (+) 621 WP_272548926.1 dephospho-CoA kinase -
  M5C98_RS18585 (M5C98_18585) zapD 4072626..4073381 (+) 756 WP_272548927.1 cell division protein ZapD -
  M5C98_RS18590 (M5C98_18590) - 4073395..4073643 (+) 249 WP_272548928.1 DNA gyrase inhibitor YacG -
  M5C98_RS18595 (M5C98_18595) - 4073696..4074127 (-) 432 WP_272548929.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62735.19 Da        Isoelectric Point: 5.9286

>NTDB_id=688220 M5C98_RS18565 WP_272553337.1 4068082..4069812(+) (pilB) [Acidovorax sp. NCPPB 3576]
MAVDTAPKEGAAIALPGLGRALMSAGKLSQKSAEDIFRKSQAGKTSFIAELTGSGAVSSAELAHTVSAVFGAPLLDLEAI
DPLRIPKDLLDPKICIAYRVVALSKRNNRLIVATADPTDQEAAERIKFTTQMGVDWIIAEYDKLIKLIEANNKSVSESME
SLSSGGDFEFDDVKIEEAPENADSANDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIA
IKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKARL
MHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPDI
IMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKAPADIPHE
TLLESGYKEDEIDGTWVTYRPVGCSACNNGYKGRLGIYQVMPISEEIQRIILRDGSALEISKQAKAEGVRSLRDAGLHKA
KMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=688220 M5C98_RS18565 WP_272553337.1 4068082..4069812(+) (pilB) [Acidovorax sp. NCPPB 3576]
ATGGCTGTTGATACCGCACCCAAAGAAGGCGCCGCAATTGCCTTGCCTGGCCTAGGCCGGGCTTTGATGTCAGCCGGCAA
GCTGAGTCAAAAATCAGCTGAAGACATCTTTCGCAAATCGCAGGCGGGGAAAACCAGCTTCATTGCTGAACTCACAGGGT
CTGGAGCCGTGTCTTCAGCCGAGTTGGCGCACACGGTCTCTGCCGTGTTCGGTGCGCCTTTACTGGATCTGGAAGCGATT
GACCCCCTCCGGATTCCTAAAGATCTGCTTGATCCAAAGATCTGCATTGCCTATCGCGTCGTTGCTCTGAGCAAGCGCAA
CAATCGGCTGATTGTGGCGACGGCTGACCCTACTGACCAAGAAGCAGCAGAGAGGATCAAATTCACGACCCAGATGGGCG
TGGATTGGATTATTGCGGAATATGACAAGCTTATCAAACTTATAGAGGCTAATAATAAGTCGGTAAGTGAATCGATGGAA
AGTCTGTCGAGCGGGGGAGATTTCGAATTTGACGATGTCAAGATTGAAGAGGCTCCCGAAAATGCAGACAGCGCCAACGA
TGTTGAAGATGCGCCTATCGTCAAGTTCCTCCATAAAATGCTTTTGGACGCGTTCAATATGCGTGCTTCCGATTTGCATT
TTGAACCTTACGAACACCAATACCGGGTACGCTTTCGCATCGATGGCGAACTACGCGAAATTGCTTCGCCACCAATTGCC
ATCAAGGACAAGCTTGCGTCCCGAATCAAGGTCATTTCCCGGCTTGATATTTCGGAAAAACGTGTGCCACAAGATGGCCG
TATGAAACTGAAAGTCGGCCCGGATCGGGTCATCGACTTTCGGGTGAGTACGCTGCCAACGCTCTTTGGTGAAAAGATCG
TGATTCGGATTCTGGATCCTAGCAGCGCCAAGTTGGGCATTGATGCGCTAGGTTACGAGCCAGAAGAAAAGGCACGACTG
ATGCATGCGATAGGACGACCTTATGGCATGATTTTGGTTACCGGACCAACTGGCTCCGGAAAAACCGTTTCTTTGTACAC
CTGCCTGAACTTGCTCAATAAGGCGGGAGTCAATATTGCCACCGCTGAGGACCCTTCGGAAATCAATTTACCTGGTGTTA
ATCAGGTAAACGTTAATGAGAAGGCGGGACTAACCTTTGCTGTCGCACTCAAATCGTTTCTGCGTCAAGATCCTGACATC
ATCATGGTCGGCGAAATTCGCGACCTTGAAACAGCGGATATTTCCATTAAGGCTGCCCAGACGGGCCACTTGGTGCTGTC
CACCCTACATACCAATGATGCGCCAACCACGCTCACCCGGATGCGCAATATGGGAATTGCGCCATTCAATATTGCGTCAA
GCGTCATTCTGATAACAGCCCAGCGCTTGGCTCGCCGTTTATGCCCCGTCTGCAAAGCGCCTGCGGATATTCCACATGAA
ACCTTGCTCGAATCCGGCTACAAAGAGGATGAGATCGATGGTACTTGGGTGACATATCGTCCGGTGGGTTGCTCTGCTTG
CAACAATGGCTACAAAGGACGACTGGGTATTTATCAAGTCATGCCAATTTCCGAAGAAATTCAACGCATTATTCTGCGGG
ATGGAAGTGCATTGGAAATCTCCAAGCAAGCCAAAGCGGAGGGGGTCCGCTCCTTACGCGATGCAGGTCTTCATAAAGCC
AAGATGGGGCTAACCTCTCTCGAAGAGGTGCTGGCCGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.059

100

0.543
  pilB Acinetobacter baylyi ADP1

53.596

100

0.543
  pilF Neisseria gonorrhoeae MS11

52.482

97.917

0.514
  pilB Legionella pneumophila strain ERS1305867

49.912

98.09

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

45.503

98.438

0.448
  pilB Vibrio campbellii strain DS40M4

45.293

97.743

0.443
  pilB Vibrio cholerae strain A1552

44.938

97.743

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.721

99.653

0.396
  pilF Thermus thermophilus HB27

38.772

98.958

0.384