Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PDPUS_RS13280 Genome accession   NZ_AP018045
Coordinates   2724102..2725208 (-) Length   368 a.a.
NCBI ID   WP_086957245.1    Uniprot ID   A0A1V1V6D8
Organism   Photobacterium damselae subsp. piscicida strain 91-197     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719102..2730208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDPUS_RS13250 - 2719664..2719969 (+) 306 Protein_2505 SprT-like domain-containing protein -
  PDPUS_RS13255 (PDPUS_1_02778) endA 2720100..2720804 (+) 705 WP_086957243.1 deoxyribonuclease I -
  PDPUS_RS13260 (PDPUS_1_02779) rsmE 2720936..2721667 (+) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PDPUS_RS13265 (PDPUS_1_02780) gshB 2721688..2722635 (+) 948 WP_086957244.1 glutathione synthase -
  PDPUS_RS13270 (PDPUS_1_02781) - 2722789..2723352 (+) 564 WP_044174430.1 YqgE/AlgH family protein -
  PDPUS_RS13275 (PDPUS_1_02782) ruvX 2723349..2723774 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  PDPUS_RS13280 (PDPUS_1_02783) pilU 2724102..2725208 (-) 1107 WP_086957245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PDPUS_RS13285 (PDPUS_1_02784) - 2725220..2726254 (-) 1035 Protein_2512 type IV pilus twitching motility protein PilT -
  PDPUS_RS13290 (PDPUS_1_02786) - 2726277..2726978 (+) 702 WP_086957246.1 YggS family pyridoxal phosphate-dependent enzyme -
  PDPUS_RS13295 (PDPUS_1_02787) proC 2726994..2727812 (+) 819 WP_086957247.1 pyrroline-5-carboxylate reductase -
  PDPUS_RS13300 (PDPUS_1_02788) - 2727824..2728381 (+) 558 WP_005297682.1 YggT family protein -
  PDPUS_RS13305 (PDPUS_1_02789) yggU 2728378..2728677 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  PDPUS_RS13310 (PDPUS_1_02790) - 2728779..2729372 (+) 594 WP_044174415.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41368.53 Da        Isoelectric Point: 5.5903

>NTDB_id=68813 PDPUS_RS13280 WP_086957245.1 2724102..2725208(-) (pilU) [Photobacterium damselae subsp. piscicida strain 91-197]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGETLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=68813 PDPUS_RS13280 WP_086957245.1 2724102..2725208(-) (pilU) [Photobacterium damselae subsp. piscicida strain 91-197]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGAGTGTTGCATCCCATTGGCGAGACGTTAGATCGTACTGGGGTTGATTTGCTATTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTACGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGGCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACGAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTTACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V1V6D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.392

99.728

0.682
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

93.75

0.391
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment