Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PALA54_RS25990 Genome accession   NZ_CP111032
Coordinates   5549672..5550211 (-) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5544672..5555211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA54_RS25970 (PALA54_05139) - 5545357..5547612 (-) 2256 WP_034027606.1 DUF1631 domain-containing protein -
  PALA54_RS25975 (PALA54_05140) nadC 5547898..5548746 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA54_RS25985 (PALA54_05142) - 5548910..5549467 (-) 558 WP_224754157.1 hypothetical protein -
  PALA54_RS25990 pilE 5549672..5550211 (-) 540 WP_033938465.1 pilin Machinery gene
  PALA54_RS25995 (PALA54_05143) pilB 5550440..5552143 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA54_RS26000 (PALA54_05144) - 5552147..5552611 (+) 465 Protein_5151 type II secretion system F family protein -
  PALA54_RS26005 (PALA54_05145) - 5552641..5553669 (-) 1029 WP_041110544.1 IS30-like element ISPsp4 family transposase -
  PALA54_RS26010 pilC 5553616..5554479 (+) 864 WP_269973640.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=686388 PALA54_RS25990 WP_033938465.1 5549672..5550211(-) (pilE) [Pseudomonas aeruginosa strain PALA54]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=686388 PALA54_RS25990 WP_033938465.1 5549672..5550211(-) (pilE) [Pseudomonas aeruginosa strain PALA54]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363