Detailed information NTG0684500

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PALA47_RS24405
• Genome accession: NZ_CP110352
• Coordinates: 5272758..5274458 (+)
• Length: 566 a.a.
• NCBI ID: WP_003107297.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PALA47
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5267758..5279458

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA47_RS24375 (PALA47_04829)	nadC	5268412..5269260 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PALA47_RS24385 (PALA47_04831)	-	5269441..5270799 (-)	1359	WP_226342803.1	O-antigen ligase family protein	-
PALA47_RS24390	-	5270891..5271205 (-)	315	WP_269972583.1	pilin	-
PALA47_RS24395 (PALA47_04832)	-	5271276..5272256 (-)	981	WP_003117291.1	IS5-like element ISPa41 family transposase	-
PALA47_RS24400 (PALA47_04833)	-	5272348..5272527 (-)	180	Protein_4832	pilin	-
PALA47_RS24405 (PALA47_04834)	pilB	5272758..5274458 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PALA47_RS24410 (PALA47_04835)	pilC	5274462..5275679 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
PALA47_RS24415 (PALA47_04836)	pilD	5275680..5276552 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PALA47_RS24420 (PALA47_04837)	coaE	5276549..5277160 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
PALA47_RS24425 (PALA47_04838)	yacG	5277157..5277357 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PALA47_RS24430 (PALA47_04839)	-	5277394..5277603 (-)	210	WP_020750398.1	hypothetical protein	-
PALA47_RS24435 (PALA47_04840)	-	5277709..5278398 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
PALA47_RS24440 (PALA47_04841)	-	5278395..5278865 (-)	471	WP_003103866.1	hypothetical protein	-
PALA47_RS24445 (PALA47_04842)	-	5278862..5279287 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62312.21 Da
• Isoelectric Point: 5.4470

>NTDB_id=684500 PALA47_RS24405 WP_003107297.1 5272758..5274458(+) (pilB) [Pseudomonas aeruginosa strain PALA47]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=684500 PALA47_RS24405 WP_003107297.1 5272758..5274458(+) (pilB) [Pseudomonas aeruginosa strain PALA47]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (190-457)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.79	100	0.569
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.521	98.763	0.509
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.158	94.17	0.491
pilB	Vibrio campbellii strain DS40M4	51.22	94.17	0.482
pilB/pilB1	Synechocystis sp. PCC 6803	37.763	100	0.412
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.727	97.173	0.376