Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PALA44_RS31035 Genome accession   NZ_CP110350
Coordinates   6623989..6624528 (-) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6618989..6629528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA44_RS32295 (PALA44_06126) - 6619581..6620132 (-) 552 Protein_6131 RHS repeat protein -
  PALA44_RS31015 - 6621130..6621282 (-) 153 Protein_6132 hypothetical protein -
  PALA44_RS31020 (PALA44_06127) - 6622742..6622984 (+) 243 WP_023109353.1 hypothetical protein -
  PALA44_RS31030 (PALA44_06129) - 6623227..6623925 (-) 699 WP_223677410.1 hypothetical protein -
  PALA44_RS31035 pilE 6623989..6624528 (-) 540 WP_033938465.1 pilin Machinery gene
  PALA44_RS31040 (PALA44_06130) pilB 6624757..6626460 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA44_RS31045 (PALA44_06131) pilC 6626464..6627681 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PALA44_RS31050 (PALA44_06132) pilD 6627684..6628556 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA44_RS31055 (PALA44_06133) coaE 6628553..6629164 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA44_RS31060 (PALA44_06134) yacG 6629161..6629361 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=684325 PALA44_RS31035 WP_033938465.1 6623989..6624528(-) (pilE) [Pseudomonas aeruginosa strain PALA44]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=684325 PALA44_RS31035 WP_033938465.1 6623989..6624528(-) (pilE) [Pseudomonas aeruginosa strain PALA44]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363