Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA52_RS21890 Genome accession   NZ_CP110347
Coordinates   4741772..4743181 (-) Length   469 a.a.
NCBI ID   WP_024916441.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4736772..4748181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA52_RS21880 (PALA52_04347) lapA 4738958..4740064 (-) 1107 WP_263957696.1 alkaline phosphatase LapA -
  PALA52_RS21885 (PALA52_04348) pilC 4740558..4741772 (-) 1215 WP_024916440.1 type II secretion system inner membrane protein GspF Machinery gene
  PALA52_RS21890 (PALA52_04349) pilB 4741772..4743181 (-) 1410 WP_024916441.1 type II secretion system ATPase GspE Machinery gene
  PALA52_RS21895 (PALA52_04350) gspD 4743178..4745589 (-) 2412 WP_270124886.1 type II secretion system secretin GspD -
  PALA52_RS21900 (PALA52_04351) - 4745586..4746173 (-) 588 WP_024916443.1 type II secretion system protein M -
  PALA52_RS21905 (PALA52_04352) gspL 4746166..4747371 (-) 1206 WP_024916444.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50966.35 Da        Isoelectric Point: 5.7943

>NTDB_id=683988 PALA52_RS21890 WP_024916441.1 4741772..4743181(-) (pilB) [Pseudomonas aeruginosa strain PALA52]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
ADLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=683988 PALA52_RS21890 WP_024916441.1 4741772..4743181(-) (pilB) [Pseudomonas aeruginosa strain PALA52]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGTACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GCCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACCGACCTGCTCGACACCCAGGATGGCGCGCCGGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGACGAGGCCAGCGACATCCATATCGAACCCTTCGAGACGCACTCGG
TGGTGCGCTACCGGGTCGATGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTATCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGTCTGCCCCAGGACGGTCGTATCGCCCTGCGGGTGGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCATGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCGCTGGCACGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACCCGGCTGGTGGACATGGGCGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTCGGCGTGCTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGTCCGGCCTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAGCTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGACCTCCGCGAAGCCGCCCGCGCCGCCGGTATGCGCAGCATGCGCGAGGACGGCGAGCGTTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACACGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373
  pilB Acinetobacter baumannii D1279779

44.359

83.156

0.369