Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA35_RS10155 Genome accession   NZ_CP110346
Coordinates   2132715..2134223 (+) Length   502 a.a.
NCBI ID   WP_078451543.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2127715..2139223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA35_RS10135 (PALA35_02016) - 2127745..2128956 (+) 1212 WP_019486563.1 O-succinylhomoserine sulfhydrylase -
  PALA35_RS10140 (PALA35_02017) - 2128953..2129720 (+) 768 WP_023106180.1 SDR family oxidoreductase -
  PALA35_RS10145 (PALA35_02018) xcpQ 2129807..2131783 (-) 1977 WP_023106181.1 GspD family T2SS secretin variant XcpQ -
  PALA35_RS10150 (PALA35_02019) xcpP 2131788..2132495 (-) 708 WP_042173362.1 type II secretion system protein N -
  PALA35_RS10155 (PALA35_02020) pilB 2132715..2134223 (+) 1509 WP_078451543.1 GspE family T2SS ATPase variant XcpR Machinery gene
  PALA35_RS10160 (PALA35_02021) xcpS 2134223..2135440 (+) 1218 WP_269974894.1 GspF family T2SS innner membrane protein variant XcpS -
  PALA35_RS10165 (PALA35_02022) xcpT 2135445..2135891 (+) 447 WP_003130409.1 GspG family T2SS major pseudopilin variant XcpT -
  PALA35_RS10170 (PALA35_02023) xcpU 2135898..2136416 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  PALA35_RS10175 (PALA35_02024) xcpV 2136413..2136805 (+) 393 WP_023125591.1 GspI family T2SS minor pseudopilin variant XcpV -
  PALA35_RS10180 (PALA35_02025) xcpW 2136802..2137515 (+) 714 WP_023108526.1 GspJ family T2SS minor pseudopilin variant XcpW -
  PALA35_RS10185 (PALA35_02026) xcpX 2137512..2138513 (+) 1002 WP_003091371.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55538.76 Da        Isoelectric Point: 6.2078

>NTDB_id=683855 PALA35_RS10155 WP_078451543.1 2132715..2134223(+) (pilB) [Pseudomonas aeruginosa strain PALA35]
MMTAPLPEIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=683855 PALA35_RS10155 WP_078451543.1 2132715..2134223(+) (pilB) [Pseudomonas aeruginosa strain PALA35]
ATGATGACAGCCCCGTTACCCGAAATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGTTTCGCCAAGCGCCA
GGGTCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGATGCCTTCAACCAGGCCCTGGCGGCGTCCTAC
CAGCACGATTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCCCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCTTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTCGAACCCAAGCGCGAGCTGGCGGCCTTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAACGCATTCCCCAGGATGGACGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGCGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACCGTGCGTAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGTTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAAGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGCGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGGGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGTGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367