Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   M1F45_RS08610 Genome accession   NZ_CP096540
Coordinates   1727444..1727941 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain RIVM_M044329     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1722444..1732941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F45_RS08585 (M1F45_08570) gcvPB 1722606..1724078 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  M1F45_RS08590 (M1F45_08575) gcvPA 1724071..1725417 (-) 1347 WP_021285939.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  M1F45_RS08595 (M1F45_08580) gcvT 1725437..1726528 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  M1F45_RS08600 (M1F45_08585) - 1726687..1727211 (-) 525 WP_001015120.1 shikimate kinase -
  M1F45_RS08605 (M1F45_08590) - 1727201..1727347 (-) 147 WP_001789879.1 hypothetical protein -
  M1F45_RS08610 (M1F45_08595) comGF 1727444..1727941 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F45_RS08615 (M1F45_08600) comGE 1727859..1728158 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F45_RS08620 (M1F45_08605) comGD 1728145..1728591 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F45_RS08625 (M1F45_08610) comGC 1728569..1728880 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F45_RS08630 (M1F45_08615) comGB 1728894..1729964 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F45_RS08635 (M1F45_08620) comGA 1729936..1730910 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F45_RS08640 (M1F45_08625) - 1730962..1731585 (-) 624 WP_021285938.1 MBL fold metallo-hydrolase -
  M1F45_RS08645 (M1F45_08630) - 1731582..1731911 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F45_RS08650 (M1F45_08635) - 1731911..1732897 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=681736 M1F45_RS08610 WP_001825340.1 1727444..1727941(-) (comGF) [Staphylococcus aureus strain RIVM_M044329]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=681736 M1F45_RS08610 WP_001825340.1 1727444..1727941(-) (comGF) [Staphylococcus aureus strain RIVM_M044329]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97