Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   M1F45_RS08630 Genome accession   NZ_CP096540
Coordinates   1728894..1729964 (-) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus strain RIVM_M044329     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1723894..1734964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F45_RS08590 (M1F45_08575) gcvPA 1724071..1725417 (-) 1347 WP_021285939.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  M1F45_RS08595 (M1F45_08580) gcvT 1725437..1726528 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  M1F45_RS08600 (M1F45_08585) - 1726687..1727211 (-) 525 WP_001015120.1 shikimate kinase -
  M1F45_RS08605 (M1F45_08590) - 1727201..1727347 (-) 147 WP_001789879.1 hypothetical protein -
  M1F45_RS08610 (M1F45_08595) comGF 1727444..1727941 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F45_RS08615 (M1F45_08600) comGE 1727859..1728158 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F45_RS08620 (M1F45_08605) comGD 1728145..1728591 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F45_RS08625 (M1F45_08610) comGC 1728569..1728880 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F45_RS08630 (M1F45_08615) comGB 1728894..1729964 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F45_RS08635 (M1F45_08620) comGA 1729936..1730910 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F45_RS08640 (M1F45_08625) - 1730962..1731585 (-) 624 WP_021285938.1 MBL fold metallo-hydrolase -
  M1F45_RS08645 (M1F45_08630) - 1731582..1731911 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F45_RS08650 (M1F45_08635) - 1731911..1732897 (-) 987 WP_000161315.1 ROK family glucokinase -
  M1F45_RS08655 (M1F45_08640) - 1732894..1733097 (-) 204 WP_000087559.1 YqgQ family protein -
  M1F45_RS08660 (M1F45_08645) - 1733078..1734541 (-) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=681740 M1F45_RS08630 WP_000776425.1 1728894..1729964(-) (comGB) [Staphylococcus aureus strain RIVM_M044329]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=681740 M1F45_RS08630 WP_000776425.1 1728894..1729964(-) (comGB) [Staphylococcus aureus strain RIVM_M044329]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994