Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTO69_RS11455 Genome accession   NZ_CP096199
Coordinates   2549561..2551243 (+) Length   560 a.a.
NCBI ID   WP_248329359.1    Uniprot ID   -
Organism   Vibrio sinaloensis strain YA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544561..2556243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO69_RS11425 (MTO69_11425) - 2544700..2545443 (-) 744 WP_248329349.1 thioredoxin fold domain-containing protein -
  MTO69_RS11430 (MTO69_11430) xerD 2545482..2546378 (-) 897 WP_248334469.1 site-specific tyrosine recombinase XerD -
  MTO69_RS11435 (MTO69_11435) fldB 2546543..2547064 (+) 522 WP_248329351.1 flavodoxin FldB -
  MTO69_RS11440 (MTO69_11440) ampD 2547375..2547920 (-) 546 WP_248329353.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MTO69_RS11445 (MTO69_11445) nadC 2548065..2548952 (+) 888 WP_248329355.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MTO69_RS11450 (MTO69_11450) - 2549166..2549561 (+) 396 WP_248329357.1 pilin -
  MTO69_RS11455 (MTO69_11455) pilB 2549561..2551243 (+) 1683 WP_248329359.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTO69_RS11460 (MTO69_11460) - 2551250..2552479 (+) 1230 WP_248329361.1 type II secretion system F family protein -
  MTO69_RS11465 (MTO69_11465) pilD 2552517..2553386 (+) 870 WP_248329363.1 A24 family peptidase Machinery gene
  MTO69_RS11470 (MTO69_11470) coaE 2553388..2553996 (+) 609 WP_248329365.1 dephospho-CoA kinase -
  MTO69_RS11475 (MTO69_11475) zapD 2554031..2554771 (+) 741 WP_248329367.1 cell division protein ZapD -
  MTO69_RS11480 (MTO69_11480) yacG 2554831..2555025 (+) 195 WP_248329369.1 DNA gyrase inhibitor YacG -
  MTO69_RS11485 (MTO69_11485) rplS 2555285..2555638 (-) 354 WP_045977783.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61650.52 Da        Isoelectric Point: 6.5917

>NTDB_id=680262 MTO69_RS11455 WP_248329359.1 2549561..2551243(+) (pilB) [Vibrio sinaloensis strain YA2]
MQSDLLTTLTHAKLITPAQQHQLTQAAQQSDQPLLDLLLDSTQISEATLAKHLAQVYSLPVISLSDFDYLAASRQLALNS
LLHRFHAAVIALEAQTLRLAVADPCQQELEQAFRFATGMTIELAIADWTSIKQAIQQLPCSSPSSSSHTPIQANQLSDLV
TLSADEQRDDDDLSHDNAPVSRYIQQVLLDAVARRASDIHFEPYEQTYRVRLRCDGLLIAAEQPSYQLSRRLAARLKILA
KLDIAERRLPQDGRLKLRLNHQVSVDMRVSTLPTLFGEKVVLRILDNRAMELDIDALGFNHTQKQTYLTALSKPQGMILL
TGPTGSGKTVSLYAALNHLNAEHVNIATAEDPIEISLPGINQVQVQPEIGLDFSQALRAFLRQDPDIIMVGEVRDINTAK
MAFKAAQTGHLVLATLHTNSAAQSIERLINMGIEPFNLASSLSLVIAQRLVRKLCPHCKRSDSAHTHLAMSLDLSDQEPI
FSANVQGCQHCNFGYLGRTAIYEVLPVTSVIAEAIARRATAQQLNALALEQGLQNLKQSGSEKLRSGITSLAELQRTLLV

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=680262 MTO69_RS11455 WP_248329359.1 2549561..2551243(+) (pilB) [Vibrio sinaloensis strain YA2]
ATGCAGAGCGATCTACTCACCACCCTCACTCATGCTAAGCTCATTACCCCGGCACAGCAGCATCAGTTGACGCAGGCGGC
GCAACAGAGTGATCAGCCACTTCTCGACCTTCTGCTTGATAGCACCCAGATCAGCGAAGCAACACTTGCCAAACATCTGG
CACAGGTGTATTCGCTACCGGTCATCTCACTCTCAGACTTTGACTACCTAGCAGCGAGCCGGCAACTGGCATTAAACTCA
TTGCTACACAGGTTTCATGCCGCGGTTATTGCCCTCGAAGCGCAAACCTTACGACTGGCGGTTGCTGACCCGTGCCAACA
AGAACTTGAGCAGGCATTTCGCTTTGCTACCGGCATGACCATTGAACTGGCCATTGCTGATTGGACCTCCATCAAACAAG
CGATACAGCAGTTACCCTGTTCATCGCCATCATCCTCGTCTCACACGCCTATCCAAGCGAATCAGCTGAGCGACCTAGTG
ACACTGAGCGCTGATGAACAACGAGACGACGATGATCTTAGCCACGATAACGCCCCCGTCAGTCGCTACATTCAACAAGT
GTTGCTCGACGCGGTAGCCAGACGCGCTTCTGATATTCACTTCGAACCTTATGAACAGACCTATCGAGTCCGACTGCGTT
GTGACGGCCTGCTGATCGCGGCTGAGCAACCCTCGTATCAGTTGAGCCGACGCCTCGCCGCTCGACTCAAAATTTTGGCA
AAACTCGACATCGCCGAGCGCAGATTGCCACAAGATGGTCGACTTAAACTGCGCTTAAACCATCAAGTTTCGGTCGATAT
GCGCGTCTCAACCTTACCGACCCTGTTTGGTGAAAAAGTAGTGTTGCGCATTCTTGATAATCGCGCAATGGAGCTGGATA
TCGATGCTCTAGGATTCAATCACACCCAAAAGCAGACGTATTTAACCGCACTGAGCAAACCACAAGGAATGATTTTGCTC
ACCGGCCCAACGGGAAGTGGCAAAACAGTTTCACTCTACGCCGCGCTGAATCATCTCAATGCCGAACACGTCAACATTGC
CACCGCAGAAGACCCTATCGAGATTTCCCTGCCTGGCATCAATCAAGTGCAAGTTCAGCCTGAGATTGGCCTAGACTTTA
GCCAAGCGTTGCGCGCCTTTCTACGCCAAGACCCAGACATCATTATGGTCGGTGAGGTGCGTGATATTAATACCGCGAAA
ATGGCGTTCAAAGCCGCGCAGACCGGTCACCTAGTGTTAGCGACGTTACATACTAACTCTGCTGCACAATCCATTGAACG
TTTAATCAATATGGGCATTGAACCCTTTAATCTCGCCTCCAGCCTCTCTTTGGTCATTGCTCAACGTCTGGTACGAAAGT
TGTGCCCACACTGTAAGCGAAGCGATAGCGCCCACACTCATCTCGCCATGTCGCTGGATTTATCAGACCAAGAACCTATC
TTTAGCGCTAACGTGCAGGGCTGCCAACACTGTAACTTTGGCTACCTGGGACGAACGGCAATCTATGAAGTTCTCCCAGT
GACTTCTGTCATAGCCGAAGCCATCGCTCGCCGCGCCACCGCTCAACAACTCAATGCCCTTGCCTTAGAGCAGGGCTTAC
AGAATTTAAAACAGTCTGGCAGTGAAAAGCTACGCTCTGGGATAACTAGTCTCGCTGAGCTGCAAAGAACACTACTGGTT
TAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

55.179

100

0.552
  pilB Vibrio cholerae strain A1552

54.464

100

0.545
  pilB Vibrio campbellii strain DS40M4

53.75

100

0.538
  pilB Legionella pneumophila strain ERS1305867

43.557

98.393

0.429
  pilB Acinetobacter baylyi ADP1

42.152

100

0.427
  pilB Acinetobacter baumannii D1279779

42.626

99.286

0.423
  pilF Neisseria gonorrhoeae MS11

41.741

100

0.42
  pilF Thermus thermophilus HB27

37.213

100

0.377