Detailed information NTG0679448

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MZN67_RS04705
• Genome accession: NZ_CP096116
• Coordinates: 1038674..1039738 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain DFS-2111020037
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1033674..1044738

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MZN67_RS04695 (MZN67_04670)	-	1034714..1037098 (-)	2385	WP_027266407.1	penicillin-binding protein 1A	-
MZN67_RS04700 (MZN67_04675)	ravI	1037250..1038257 (-)	1008	WP_027266406.1	Dot/Icm T4SS effector RavI	-
MZN67_RS04705 (MZN67_04680)	pilM	1038674..1039738 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
MZN67_RS04710 (MZN67_04685)	pilN	1039742..1040290 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
MZN67_RS04715 (MZN67_04690)	pilO	1040304..1040903 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
MZN67_RS04720 (MZN67_04695)	pilP	1040900..1041484 (+)	585	WP_161504771.1	pilus assembly protein PilP	Machinery gene
MZN67_RS04725 (MZN67_04700)	pilQ	1041488..1043584 (+)	2097	WP_011215098.1	type IV pilus secretin PilQ	Machinery gene
MZN67_RS04730 (MZN67_04705)	aroK	1044030..1044557 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=679448 MZN67_RS04705 WP_011215094.1 1038674..1039738(+) (pilM) [Legionella pneumophila subsp. pneumophila strain DFS-2111020037]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=679448 MZN67_RS04705 WP_011215094.1 1038674..1039738(+) (pilM) [Legionella pneumophila subsp. pneumophila strain DFS-2111020037]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGATGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAGTCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407