Detailed information NTG0679268

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MY487_RS12170
• Genome accession: NZ_CP096033
• Coordinates: 2506684..2507298 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus amyloliquefaciens strain GL18
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2501684..2512298

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MY487_RS12140 (MY487_12140)	rpsT	2501987..2502253 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MY487_RS12145 (MY487_12145)	holA	2502270..2503311 (-)	1042	Protein_2348	DNA polymerase III subunit delta	-
MY487_RS12150 (MY487_12150)	-	2503351..2503502 (-)	152	Protein_2349	hypothetical protein	-
MY487_RS12155 (MY487_12155)	-	2503543..2503677 (+)	135	WP_003152870.1	YqzM family protein	-
MY487_RS12160 (MY487_12160)	comEC	2503696..2506047 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MY487_RS12165 (MY487_12165)	-	2506048..2506617 (-)	570	WP_003152868.1	ComE operon protein 2	-
MY487_RS12170 (MY487_12170)	comEA	2506684..2507298 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
MY487_RS12175 (MY487_12175)	comER	2507357..2508178 (+)	822	WP_012118027.1	late competence protein ComER	-
MY487_RS12180 (MY487_12180)	-	2508247..2508984 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
MY487_RS12185 (MY487_12185)	rsfS	2508981..2509337 (-)	357	WP_007408260.1	ribosome silencing factor	-
MY487_RS12190 (MY487_12190)	yqeK	2509354..2509914 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MY487_RS12195 (MY487_12195)	-	2509904..2510473 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
MY487_RS12200 (MY487_12200)	yhbY	2510484..2510774 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MY487_RS12205 (MY487_12205)	aroE	2510768..2511610 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=679268 MY487_RS12170 WP_012118026.1 2506684..2507298(-) (comEA) [Bacillus amyloliquefaciens strain GL18]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=679268 MY487_RS12170 WP_012118026.1 2506684..2507298(-) (comEA) [Bacillus amyloliquefaciens strain GL18]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363