Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MY523_RS03085 Genome accession   NZ_CP096016
Coordinates   692503..694212 (-) Length   569 a.a.
NCBI ID   WP_250657343.1    Uniprot ID   -
Organism   Alkalimarinus coralli strain SCSIO 12582     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 687503..699212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY523_RS03050 - 687930..688649 (-) 720 WP_250657336.1 LrgB family protein -
  MY523_RS03055 - 688642..689004 (-) 363 WP_250657337.1 CidA/LrgA family protein -
  MY523_RS03060 - 689030..689470 (-) 441 WP_250657338.1 HIT family protein -
  MY523_RS03065 yacG 689522..689737 (-) 216 WP_250657339.1 DNA gyrase inhibitor YacG -
  MY523_RS03070 coaE 689712..690326 (-) 615 WP_250657340.1 dephospho-CoA kinase -
  MY523_RS03075 pilD 690335..691204 (-) 870 WP_250657341.1 A24 family peptidase Machinery gene
  MY523_RS03080 pilC 691280..692500 (-) 1221 WP_250657342.1 type II secretion system F family protein Machinery gene
  MY523_RS03085 pilB 692503..694212 (-) 1710 WP_250657343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MY523_RS03095 - 694548..695003 (+) 456 WP_275975288.1 pilin -
  MY523_RS03100 - 695092..697017 (+) 1926 WP_250657344.1 hypothetical protein -
  MY523_RS03105 - 697019..697921 (+) 903 WP_250657345.1 glycosyltransferase family 2 protein -
  MY523_RS03110 - 697918..699099 (+) 1182 WP_250657346.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62350.28 Da        Isoelectric Point: 4.7853

>NTDB_id=679133 MY523_RS03085 WP_250657343.1 692503..694212(-) (pilB) [Alkalimarinus coralli strain SCSIO 12582]
MASNSSITLTGLARKFVLDNILEEEIAKDAFAQANNNNIPFITYLVQNNLAGAKALAYSAAQEFGMPVLDLSTFMAELIP
EKVVEEKLIRKHHALPLFKRGNRLFIATSDPTNIQALDELKFHTGLSTDAVLVEDDKLGDAIEKYLESQDTSMGDLEDAD
IDGLDIENPEEEKEEQAGSEADDAPIVKYVNKMLLDAIKGGASDLHFEPYEKTYRVRYRTDGILHEVSKPSIKLASKIAA
RLKVMSQLDISERRIPQDGRIKLKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEDQKQLYLDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNPKVGLGFPEALRSFLRQDPDIVMVGEIR
DLDTASIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSACKEKANVPKETLLKEGF
TEEQIETGFTLYQPKGCDKCSGGYKGRVGIYEVVKVTPAISQLIMEEATSLDIAAAAQKEGFNNLRQSAILKAIEGVTSL
EEVNRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=679133 MY523_RS03085 WP_250657343.1 692503..694212(-) (pilB) [Alkalimarinus coralli strain SCSIO 12582]
ATGGCATCTAATAGTTCAATCACACTAACTGGTTTGGCTCGCAAATTTGTTTTGGACAATATTCTCGAAGAAGAGATAGC
TAAAGACGCATTTGCTCAAGCAAACAATAACAATATCCCTTTTATTACTTACTTGGTGCAAAACAATCTAGCTGGCGCTA
AAGCACTTGCATATTCTGCAGCGCAGGAATTCGGGATGCCGGTTCTCGACCTATCGACTTTTATGGCAGAATTGATTCCT
GAAAAAGTAGTAGAAGAGAAGTTAATAAGAAAACATCATGCACTCCCTCTGTTCAAGCGTGGCAATCGGCTATTTATAGC
AACTTCAGACCCTACCAACATTCAAGCCCTCGATGAACTTAAGTTCCACACTGGGTTAAGCACCGACGCAGTACTGGTTG
AAGACGACAAACTAGGTGATGCAATAGAAAAATATCTGGAATCTCAAGATACGTCAATGGGCGATCTTGAAGATGCAGAT
ATTGATGGTCTTGATATTGAAAACCCAGAAGAAGAAAAAGAGGAACAAGCTGGATCAGAAGCTGATGACGCCCCTATCGT
AAAATACGTAAATAAGATGCTTCTTGACGCAATAAAAGGCGGAGCATCAGACCTACATTTTGAGCCCTATGAAAAAACTT
ACCGAGTCAGATACAGGACTGACGGCATTCTTCATGAGGTATCAAAACCTTCAATTAAATTAGCGTCTAAGATAGCTGCG
CGCTTAAAAGTCATGTCACAACTTGATATTTCTGAACGAAGAATCCCTCAAGATGGCCGAATCAAGCTAAAGCTATCCAA
AACGAAAGCCATCGACTTCCGGGTAAATACACTCCCTACACTCTGGGGAGAAAAAATCGTACTGCGTATACTTGACCCAA
GCAGTGCTAAAATGGGGATAGATGCACTCGGCTATGAGGAAGATCAAAAGCAACTCTACCTGGATGCGCTTGCCCAGCCG
CAAGGTATGATTTTGGTGACAGGGCCTACAGGTAGCGGAAAAACAGTATCGCTCTATACCGGTCTTAATATACTTAACAC
GCCTGAAACCAATATATCCACAGCAGAAGACCCTGTCGAAATCAACCTTGAAGGTATTAATCAGGTCAACGTCAACCCCA
AGGTGGGGTTAGGCTTCCCTGAGGCTCTAAGATCATTTCTACGGCAAGATCCGGATATCGTCATGGTGGGGGAGATTCGA
GATTTAGATACCGCTTCAATAGCGATCAAAGCCGCTCAAACGGGGCACCTGGTGCTCTCTACACTACACACAAACAGTGC
GCCTGAAACACTCACCCGAATGATGAATATGGGCGTTCCTGCTTTTAACATTGCAACATCTGTCAGTCTGATTATTGCAC
AGCGTTTAGGCAGAAGACTGTGTAGTGCATGCAAAGAAAAAGCTAACGTCCCCAAAGAAACCCTTTTAAAGGAGGGGTTC
ACAGAAGAACAAATTGAAACAGGCTTTACATTGTACCAACCGAAAGGCTGTGATAAATGTTCAGGTGGATATAAAGGGCG
AGTCGGTATTTACGAAGTCGTTAAAGTGACACCAGCAATATCTCAGCTAATTATGGAAGAAGCTACCTCCCTTGATATAG
CCGCCGCAGCACAAAAAGAAGGCTTTAACAATTTGAGACAGTCTGCAATTCTAAAAGCAATTGAAGGCGTAACTAGCTTG
GAAGAAGTTAACCGAGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.402

100

0.594
  pilB Acinetobacter baylyi ADP1

57.473

98.77

0.568
  pilB Legionella pneumophila strain ERS1305867

56.36

99.473

0.561
  pilF Neisseria gonorrhoeae MS11

49.381

99.297

0.49
  pilB Vibrio cholerae strain A1552

52.941

92.619

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

50.735

95.606

0.485
  pilB Vibrio campbellii strain DS40M4

53.15

89.279

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.055

93.322

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

40.038

93.497

0.374