Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MW889_RS18130 Genome accession   NZ_CP095867
Coordinates   3833087..3834799 (-) Length   570 a.a.
NCBI ID   WP_031952279.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain 11J360     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3828087..3839799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW889_RS18100 (MW889_18100) - 3828251..3828577 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  MW889_RS18105 (MW889_18105) rlmB 3828679..3829428 (+) 750 WP_002156969.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MW889_RS18110 (MW889_18110) - 3829464..3830378 (+) 915 WP_033846385.1 DMT family transporter -
  MW889_RS18115 (MW889_18115) coaE 3830375..3830971 (-) 597 WP_004883551.1 dephospho-CoA kinase -
  MW889_RS18120 pilD 3830973..3831833 (-) 861 WP_022575844.1 A24 family peptidase Machinery gene
  MW889_RS18125 (MW889_18130) pilC 3831833..3833059 (-) 1227 WP_033846386.1 type II secretion system F family protein Machinery gene
  MW889_RS18130 (MW889_18135) pilB 3833087..3834799 (-) 1713 WP_031952279.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MW889_RS18135 (MW889_18140) tpiA 3835090..3835884 (+) 795 WP_004710827.1 triose-phosphate isomerase -
  MW889_RS18140 (MW889_18145) secG 3835897..3836226 (+) 330 WP_002156913.1 preprotein translocase subunit SecG -
  MW889_RS18160 (MW889_18165) rimP 3836863..3837387 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  MW889_RS18165 (MW889_18170) nusA 3837425..3838909 (+) 1485 WP_002156952.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63469.90 Da        Isoelectric Point: 5.2413

>NTDB_id=678901 MW889_RS18130 WP_031952279.1 3833087..3834799(-) (pilB) [Acinetobacter nosocomialis strain 11J360]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEEIPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=678901 MW889_RS18130 WP_031952279.1 3833087..3834799(-) (pilB) [Acinetobacter nosocomialis strain 11J360]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCCACCAGCAATCCAAGTAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAATACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGACGTATTAAACTTAAGCTGTCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACCTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCCCTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAAGCTGCTCAAACCGGCCATATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTTTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCGGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGTTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.596

100

0.986
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.579

90.877

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365